	US 11,789,011 B2
	Engineered three-dimensional breast tissue, adipose tissue, and tumor disease model
Shelby Marie King, San Diego, CA (US); Deborah Lynn Greene Nguyen, San Diego, CA (US); Vivian A. Gorgen, San Diego, CA (US); Benjamin R. Shepherd, San Diego, CA (US); Sharon C. Presnell, San Diego, CA (US); Rosalie Sears, Portland, OR (US); Brittany Allen-Petersen, Portland, OR (US); and Ellen Langer, Portland, OR (US)
Filed by Organovo, Inc., San Diego, CA (US); and Oregon Health & Science University, Portland, OR (US)
Filed on Dec. 12, 2019, as Appl. No. 16/712,645.
Application 15/911,713 is a division of application No. 14/678,392, filed on Apr. 3, 2015, granted, now 9,983,195, issued on May 29, 2018.
Application 16/712,645 is a continuation of application No. 15/911,713, filed on Mar. 5, 2018, abandoned.
Claims priority of provisional application 61/976,895, filed on Apr. 8, 2014.
Claims priority of provisional application 61/975,640, filed on Apr. 4, 2014.
Prior Publication US 2020/0116702 A1, Apr. 16, 2020
Int. Cl. C12N 5/071 (2010.01); G01N 33/50 (2006.01); C12N 5/09 (2010.01); B33Y 10/00 (2015.01); B33Y 80/00 (2015.01); C12N 5/077 (2010.01)

CPC G01N 33/5011 (2013.01) [B33Y 10/00 (2014.12); B33Y 80/00 (2014.12); C12N 5/0656 (2013.01); C12N 5/0693 (2013.01); C12N 5/0697 (2013.01); G01N 33/5082 (2013.01); C12N 2502/13 (2013.01); C12N 2502/1305 (2013.01); C12N 2502/1323 (2013.01); C12N 2502/1358 (2013.01); C12N 2502/28 (2013.01); C12N 2513/00 (2013.01); C12N 2533/74 (2013.01)]

23 Claims

1. A culture comprising a three-dimensional, engineered, biological breast cancer model and a cell culture media,

wherein the breast cancer model comprises:

a. breast stromal tissue comprising human mammary fibroblasts, human endothelial cells, and human mesenchymal stem cells; and

b. breast cancer tumor tissue comprising breast cancer cells and human endothelial cells, the tumor tissue surrounded on all sides by the stromal tissue to form the three-dimensional, engineered, biological breast cancer model;

wherein the cell culture media comprises an adipocyte differentiation signal for differentiation of the human mesenchymal stem cells in the breast cancer model into human adipocytes, and

wherein the human mesenchymal stem cells in the breast cancer model that are exposed to the adipocyte differentiation signal are capable of differentiating into adipocytes in the culture.