CPC C12Q 1/689 (2013.01) [A61K 35/747 (2013.01); C12Q 2600/106 (2013.01); C12Q 2600/16 (2013.01)] | 20 Claims |
1. A method for preparing an amplified lung microbiome from a subject, comprising
(a) obtaining a bronchoalveolar lavage (BAL) sample from the subject;
(b) culturing a first portion of the BAL sample in a liquid Tryptic Soy broth medium under conditions suitable to expand a first aerobic lung bacteria population;
(c) culturing a second portion of the BAL sample in a liquid Lactobacilli-adapted MRS broth medium under conditions suitable to expand a second aerobic lung bacteria population; and
(d) culturing a third portion of the BAL sample in a modified liquid Chopped Meat medium supplemented with DHNA (1,4-dihydroxy-2-naphthoic acid) under conditions suitable to expand a first anaerobic lung bacteria population,
(e) mixing the first aerobic lung bacteria population, second aerobic lung bacteria population, and the first anaerobic lung bacteria population of steps (b)-(d) produces the amplified lung microbiome, wherein the amplified lung microbiome comprises bacteria from two or more of the following bacterial classes: gammaproteobacteria, fusobacteriia, actinobacteria, bacteroidia, and deltaproteobacteria.
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