US 11,788,149 B2
Single-stranded oligonucleotide probes for chromosome or gene copy enumeration
Michael Farrell, Tucson, AZ (US); Antony Hubbard, Tucson, AZ (US); Donald Johnson, Tucson, AZ (US); Brian Kelly, Tucson, AZ (US); Taylor Shingler, Tucson, AZ (US); Lei Tang, Oro Valley, AZ (US); and Wenjun Zhang, Tucson, AZ (US)
Assigned to Ventana Medical Systems, Inc., Tucson, AZ (US)
Filed by Ventana Medical Systems, Inc., Tucson, AZ (US)
Filed on May 20, 2020, as Appl. No. 16/879,228.
Application 16/879,228 is a division of application No. 15/242,471, filed on Aug. 19, 2016, granted, now 10,752,955.
Application 15/242,471 is a continuation of application No. PCT/EP2015/053555, filed on Feb. 20, 2015.
Claims priority of provisional application 62/094,543, filed on Dec. 19, 2014.
Claims priority of provisional application 61/943,196, filed on Feb. 21, 2014.
Prior Publication US 2021/0002726 A1, Jan. 7, 2021
Int. Cl. C12Q 1/6886 (2018.01); C12Q 1/6841 (2018.01)
CPC C12Q 1/6886 (2013.01) [C12Q 1/6841 (2013.01); C12Q 2600/156 (2013.01); C12Q 2600/158 (2013.01)] 12 Claims
 
2. A system for in situ detection of a control region of human chromosome 17, said system comprising:
a set of two or more single-stranded control probes specific for X distinct monomers of an alpha satellite control region of human chromosome 17, wherein X=2-14, the control probes are each labeled with at least one first label, wherein the control probes can achieve an enumerable signal when hybridized to chromosome 17, each enumerable signal having a generally round shape, wherein a round shape is a shape defined by a simple closed curve fitting within a first region, the first region being an area on and between an inner concentric circle and an outer concentric circle, the inner concentric circle having an inner radius (Rin) and the outer concentric circle having an outer radius (Rout) wherein Rin is ≥ 50% of Rout, and the simple closed curve having a radius Rsimple, wherein Rin≤ Rsimple≤ Rout, and wherein the simple closed curve is a connected curve that does not cross itself and ends at the same point where it begins.