US 11,788,126 B2
Compositions, methods and kits for real time polymerase chain reaction (PCR)
Carole Bornarth, Fremont, CA (US); Michael Lau, Sunnyvale, CA (US); and Junko Stevens, Menlo Park, CA (US)
Assigned to LIFE TECHNOLOGIES CORPORATION, Carlsbad, CA (US)
Filed by LIFE TECHNOLOGIES CORPORATION, Carlsbad, CA (US)
Filed on Aug. 13, 2020, as Appl. No. 16/947,727.
Application 16/947,727 is a division of application No. 15/927,572, filed on Mar. 21, 2018, granted, now 10,767,217.
Application 15/927,572 is a division of application No. 13/918,768, filed on Jun. 14, 2013, granted, now 9,951,378, issued on Apr. 24, 2018.
Claims priority of provisional application 61/659,587, filed on Jun. 14, 2012.
Prior Publication US 2020/0370109 A1, Nov. 26, 2020
Int. Cl. C12Q 1/6848 (2018.01); C12N 9/00 (2006.01); C07K 16/00 (2006.01); C12Q 1/686 (2018.01)
CPC C12Q 1/6848 (2013.01) [C07K 16/00 (2013.01); C12N 9/00 (2013.01); C12Q 1/686 (2013.01)] 11 Claims
 
1. A method for reducing non-specific priming, comprising:
contacting a nucleic acid polymerase with a dual hot start mechanism to form a composition, under conditions suitable for the dual hot start mechanism to inhibit polymerase activity of the nucleic acid polymerase at a first temperature, and
heating the composition to a second temperature under conditions suitable to halt inhibition of the nucleic acid polymerase by the dual hot start mechanism whereby the inhibition of the polymerase activity of the nucleic acid polymerase occurs until the second temperature is reached.