CPC C12Q 1/6848 (2013.01) [C07K 16/00 (2013.01); C12N 9/00 (2013.01); C12Q 1/686 (2013.01)] | 11 Claims |
1. A method for reducing non-specific priming, comprising:
contacting a nucleic acid polymerase with a dual hot start mechanism to form a composition, under conditions suitable for the dual hot start mechanism to inhibit polymerase activity of the nucleic acid polymerase at a first temperature, and
heating the composition to a second temperature under conditions suitable to halt inhibition of the nucleic acid polymerase by the dual hot start mechanism whereby the inhibition of the polymerase activity of the nucleic acid polymerase occurs until the second temperature is reached.
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