CPC C12Q 1/6834 (2013.01) [C12Q 2565/537 (2013.01)] | 22 Claims |
1. A method for counting circularized nucleic acids on a solid support, comprising:
a) forming at least one complex comprising an oligonucleotide primer hybridized to a circularized nucleic acid, wherein the primer is bound to a surface on a solid support;
b) detecting formation of the at least one complex in a process comprising:
i) extending the primer in the complex in a rolling circle amplification (RCA) reaction to form RCA product, wherein forming the RCA product comprises extending the primer bound to the surface on the solid support on the circularized nucleic acid in a reaction mixture comprising at least 12% PEG, wherein the PEG has an average molecular weight between 400 and 800;
ii) hybridizing a plurality of labeled probes to the RCA product; and
iii) detecting hybridized labeled probe;
wherein hybridized labeled probe at a locus on the surface on the solid support is indicative of formation of a complex comprising an oligonucleotide primer hybridized to a circularized nucleic acid at that locus on the solid support.
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