	US 11,788,111 B2
	Compositions and methods for making selenocysteine containing polypeptides
Dieter Soll, Guilford, CT (US); Takahito Mukai, New Haven, CT (US); and Kyle Hoffman, New Haven, CT (US)
Assigned to YALE UNIVERSITY, New Haven, CT (US)
Appl. No. 16/754,130
Filed by Yale University, New Haven, CT (US)
PCT Filed Oct. 4, 2018, PCT No. PCT/US2018/054437 § 371(c)(1), (2) Date Apr. 6, 2020, PCT Pub. No. WO2019/071023, PCT Pub. Date Apr. 11, 2019.
Prior Publication US 2020/0332336 A1, Oct. 22, 2020
Int. Cl. C12P 21/02 (2006.01); C12N 15/11 (2006.01)

CPC C12P 21/02 (2013.01) [C12N 15/111 (2013.01); C12Y 209/01001 (2013.01)]

19 Claims

1. A non-naturally occurring tRNA^Seccomprising sequence elements from a yeast tRNA^Serin combination with sequence elements from a non-yeast tRNA,

wherein the non-yeast tRNA is an Aeromonas salmonicida tRNA,

wherein the sequence of the non-naturally occurring tRNA^Seccomprises at least 85% sequence identity to the chimeric sequences of any of SEQ ID NO: 152, 154, 156 or SEQ ID NO: 158, and

wherein the non-naturally occurring tRNA^Secis a substrate for a SerRS and a SelA, and binds to eEF1α.