CPC C12P 7/04 (2013.01) [C12P 39/00 (2013.01)] | 26 Claims |
1. A method for producing at least one target product from carbon monoxide (CO) and optionally hydrogen (H2), or from carbon dioxide (CO2) and H2, the method comprising:
(i) introducing a gaseous substrate comprising at least CO and optionally H2, or at least CO2 and H2 into a first bioreactor containing a culture of at least one first microorganism in a first liquid nutrient medium and anaerobically fermenting the gaseous substrate to produce at least one first product selected from the group consisting of ethanol, lactate, and 2,3-butanediol in a first fermentation broth, wherein the first microorganism is a Wood-Ljungdahl microorganism; and
(ii) transferring at least a portion of the first fermentation broth to a second bioreactor containing a culture of at least one second microorganism in a second liquid nutrient medium, wherein the second microorganism is a different species from the first microorganism, and aerobically fermenting the first product to produce at least a first target product in a second fermentation broth; wherein the at least one second microorganism is selected from the group consisting of yeasts, Escherichia coli, Geobacillus thermoglucosidasius, Pseudomonas putida KT2440, Cupriavidus necator/Ralstonia eutropha/Alcaligenes eutrophus, Desulfonatronum cooperativum, Geoalkalibacter ferrihydriticus, Alkalispirillum mobile, and Corynebacterium glutamicum;
wherein the first target product is not a lipid.
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