US 11,788,103 B2
Secondary acetate fermentation
Sean Dennis Simpson, Skokie, IL (US); Allan Haiming Gao, Skokie, IL (US); Robert John Conrado, Skokie, IL (US); James Daniel Winkler, Skokie, IL (US); Alexander Paul Mueller, Skokie, IL (US); Steven Brown, Skokie, IL (US); Loan Phuong Tran, Skokie, IL (US); Michael Koepke, Skokie, IL (US); and Christophe Daniel Mihalcea, Skokie, IL (US)
Assigned to LanzaTech, Inc., Skokie, IL (US)
Filed by LanzaTech, Inc., Skokie, IL (US)
Filed on Jul. 24, 2020, as Appl. No. 16/938,787.
Claims priority of provisional application 62/878,528, filed on Jul. 25, 2019.
Claims priority of provisional application 62/986,940, filed on Mar. 9, 2020.
Prior Publication US 2021/0024961 A1, Jan. 28, 2021
Int. Cl. C12P 7/04 (2006.01); C12P 39/00 (2006.01)
CPC C12P 7/04 (2013.01) [C12P 39/00 (2013.01)] 26 Claims
 
1. A method for producing at least one target product from carbon monoxide (CO) and optionally hydrogen (H2), or from carbon dioxide (CO2) and H2, the method comprising:
(i) introducing a gaseous substrate comprising at least CO and optionally H2, or at least CO2 and H2 into a first bioreactor containing a culture of at least one first microorganism in a first liquid nutrient medium and anaerobically fermenting the gaseous substrate to produce at least one first product selected from the group consisting of ethanol, lactate, and 2,3-butanediol in a first fermentation broth, wherein the first microorganism is a Wood-Ljungdahl microorganism; and
(ii) transferring at least a portion of the first fermentation broth to a second bioreactor containing a culture of at least one second microorganism in a second liquid nutrient medium, wherein the second microorganism is a different species from the first microorganism, and aerobically fermenting the first product to produce at least a first target product in a second fermentation broth; wherein the at least one second microorganism is selected from the group consisting of yeasts, Escherichia coli, Geobacillus thermoglucosidasius, Pseudomonas putida KT2440, Cupriavidus necator/Ralstonia eutropha/Alcaligenes eutrophus, Desulfonatronum cooperativum, Geoalkalibacter ferrihydriticus, Alkalispirillum mobile, and Corynebacterium glutamicum;
wherein the first target product is not a lipid.