	US 11,788,098 B2
	Plant transformation
Michael Lee Nuccio, Salem, NH (US); Davide Sosso, Cambridge (IT); Tomás Cermák, Brookline (SK); and David J. Segal, Davis, CA (US)
Assigned to INARI AGRICULTURE TECHNOLOGY, INC., Cambridge, MA (US)
Filed by INARI AGRICULTURE TECHNOLOGY, INC., Cambridge, MA (US)
Filed on Mar. 8, 2022, as Appl. No. 17/654,004.
Application 17/654,004 is a continuation of application No. 16/844,438, filed on Apr. 9, 2020, granted, now 11,319,547.
Claims priority of provisional application 62/833,150, filed on Apr. 12, 2019.
Prior Publication US 2022/0195446 A1, Jun. 23, 2022
Int. Cl. C12N 15/82 (2006.01)

CPC C12N 15/8223 (2013.01)

12 Claims

1. A maize plant cell comprising at least one exogenous gene transcription agent that comprises the polypeptide of SEQ ID NO:93 and stimulates transcription of the endogenous WUS2 gene, wherein expression of the endogenous WUS2 polypeptide is increased in comparison to the expression of the endogenous WUS2 polypeptide in a control maize plant cell, wherein the endogenous WUS2 polypeptide is encoded by an endogenous polynucleotide that is operably linked to an endogenous maize WUS2 promoter of SEQ ID NO:4 or an allelic variant thereof, wherein the at least one exogenous gene transcription agent comprises an artificial zinc finger (AZF) DNA binding domain polypeptide that binds to the DNA sequence of SEQ ID NO:101 in the endogenous maize WUS2 promoter of SEQ ID NO:4, and wherein the maize plant cell can form a regenerable maize plant structure.