US 11,788,088 B2
CRISPR/Cas system and method for genome editing and modulating transcription
Ryan E. Clarke, Chicago, IL (US); Bradley J. Merrill, Chicago, IL (US); Matthew S. MacDougall, Chicago, IL (US); Hannah M. Pennington, Chicago, IL (US); and Brian R. Shy, Chicago, IL (US)
Assigned to The Board of Trustees of the University of Illinois, Urbana, IL (US)
Appl. No. 16/650,496
Filed by THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS, Urbana, IL (US)
PCT Filed Sep. 21, 2018, PCT No. PCT/US2018/052211
§ 371(c)(1), (2) Date Mar. 25, 2020,
PCT Pub. No. WO2019/067322, PCT Pub. Date Apr. 4, 2019.
Claims priority of provisional application 62/563,128, filed on Sep. 26, 2017.
Claims priority of provisional application 62/563,131, filed on Sep. 26, 2017.
Claims priority of provisional application 62/563,133, filed on Sep. 26, 2017.
Prior Publication US 2020/0308581 A1, Oct. 1, 2020
Int. Cl. C12N 15/113 (2010.01); C12N 9/22 (2006.01)
CPC C12N 15/113 (2013.01) [C12N 9/22 (2013.01); C12Y 603/02019 (2013.01); C07K 2319/06 (2013.01); C07K 2319/80 (2013.01); C07K 2319/95 (2013.01); C12N 2310/121 (2013.01); C12N 2310/122 (2013.01); C12N 2310/123 (2013.01); C12N 2310/20 (2017.05)] 8 Claims
 
1. A CRISPR/Cas system comprising a Cas endonuclease and a cognate single guide RNA (sgRNA), wherein the cognate sgRNA is an activatable sgRNA harboring an inactivation sequence in a non-essential region of the sgRNA, wherein said inactivation sequence comprises one or more RNA-guided endonuclease recognition sites, and the activatable sgRNA is encoded by a nucleic acid molecule of SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21 or SEQ ID NO:22.