CPC C12N 15/113 (2013.01) [C12N 9/22 (2013.01); C12Y 603/02019 (2013.01); C07K 2319/06 (2013.01); C07K 2319/80 (2013.01); C07K 2319/95 (2013.01); C12N 2310/121 (2013.01); C12N 2310/122 (2013.01); C12N 2310/123 (2013.01); C12N 2310/20 (2017.05)] | 8 Claims |
1. A CRISPR/Cas system comprising a Cas endonuclease and a cognate single guide RNA (sgRNA), wherein the cognate sgRNA is an activatable sgRNA harboring an inactivation sequence in a non-essential region of the sgRNA, wherein said inactivation sequence comprises one or more RNA-guided endonuclease recognition sites, and the activatable sgRNA is encoded by a nucleic acid molecule of SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21 or SEQ ID NO:22.
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