US 11,788,082 B2
T4 DNA ligase variants with increased thermostability
Zhenyu Zhu, Lynnfield, MA (US); Aine Quimby, Newburyport, MA (US); Dapeng Sun, Lexington, MA (US); and Alicia DiCicco, Salem, MA (US)
Assigned to ABclonal Science, Inc., Woburn, MA (US)
Filed by AbClonal Science, Inc., Woburn, MA (US)
Filed on Feb. 22, 2022, as Appl. No. 17/677,655.
Prior Publication US 2023/0265412 A1, Aug. 24, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 9/00 (2006.01); C12P 19/34 (2006.01)
CPC C12N 9/93 (2013.01) [C12P 19/34 (2013.01); C12Y 605/01001 (2013.01)] 5 Claims
 
1. A mutant T4 DNA ligase, comprising the amino acid sequence of SEQ ID NO:6 from amino acid numbers 1 to 487.