US 11,788,077 B2
Polynucleotides encoding optimized engineered meganucleases having specificity for a recognition sequence in the Hepatitis B virus genome
James Jefferson Smith, Morrisville, NC (US); Janel Lape, Wake Forest, NC (US); Victor Bartsevich, Durham, NC (US); and Hui Li, Apex, NC (US)
Assigned to Precision BioSciences, Inc., Durham, NC (US)
Filed by Precision BioSciences, Inc., Durham, NC (US)
Filed on Sep. 20, 2021, as Appl. No. 17/479,173.
Application 17/479,173 is a division of application No. 16/381,668, filed on Apr. 11, 2019, granted, now 11,142,750.
Claims priority of provisional application 62/656,831, filed on Apr. 12, 2018.
Prior Publication US 2022/0056427 A1, Feb. 24, 2022
Int. Cl. C12N 9/22 (2006.01); C12N 15/86 (2006.01); C12N 15/85 (2006.01); A61K 47/69 (2017.01); A61K 9/16 (2006.01)
CPC C12N 9/22 (2013.01) [A61K 9/1617 (2013.01); A61K 47/6929 (2017.08); C12N 15/86 (2013.01); C12N 2015/8518 (2013.01)] 12 Claims
 
1. A polynucleotide comprising a nucleic acid sequence encoding an engineered meganuclease that recognizes and cleaves a recognition sequence consisting of SEQ ID NO: 10 within a Hepatitis B virus genome, wherein said engineered meganuclease comprises a first subunit and a second subunit, wherein said first subunit binds to a first recognition half-site of said recognition sequence and comprises a first hypervariable (HVR1) region, wherein said second subunit binds to a second recognition half-site of said recognition sequence and comprises a second hypervariable (HVR2) region, wherein said engineered meganuclease comprises the amino acid sequence of SEQ ID NO: 12 or 13.