CPC C12N 9/22 (2013.01) [A61K 9/1617 (2013.01); A61K 47/6929 (2017.08); C12N 15/86 (2013.01); C12N 2015/8518 (2013.01)] | 12 Claims |
1. A polynucleotide comprising a nucleic acid sequence encoding an engineered meganuclease that recognizes and cleaves a recognition sequence consisting of SEQ ID NO: 10 within a Hepatitis B virus genome, wherein said engineered meganuclease comprises a first subunit and a second subunit, wherein said first subunit binds to a first recognition half-site of said recognition sequence and comprises a first hypervariable (HVR1) region, wherein said second subunit binds to a second recognition half-site of said recognition sequence and comprises a second hypervariable (HVR2) region, wherein said engineered meganuclease comprises the amino acid sequence of SEQ ID NO: 12 or 13.
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