CPC C12N 9/22 (2013.01) [C12N 15/64 (2013.01); C07K 19/00 (2013.01); C07K 2319/81 (2013.01)] | 5 Claims |
1. A method of editing a T cell receptor (TCR) gene, comprising introducing into a T cell a composition comprising a nucleic acid encoding a polypeptide comprising a DNA binding domain and a functional domain, wherein
the DNA binding domain and the functional domain are connected by a polypeptide consisting of 35 to 55 amino acids,
the DNA binding domain comprises a plurality of DNA binding modules consecutively from the N-terminal side of the DNA binding domain,
a combination of the xth amino acid and the yth amino acid in the 4n-3th DNA binding module from the N-terminus being identical for any n,
a combination of the xth amino acid and the yth amino acid in the 4n-2th DNA binding module from the N-terminus being identical for any n,
a combination of the xth amino acid and the yth amino acid in the 4n-1th DNA binding module from the N-terminus being identical for any n, and
a combination of the xth amino acid and the yth amino acid in the 4nth DNA binding module from the N-terminus being identical for any n,
the combination of the xth amino acid and the yth amino acid in the 4n-3th DNA binding module from the N-terminus, the combination of the xth amino acid and the yth amino acid in the 4n-2th DNA binding module from the N-terminus, the combination of the xth amino acid and the yth amino acid in the 4n-1th DNA binding module from the N-terminus, and the combination of the xth amino acid and the yth amino acid in the 4nth DNA binding module from the N-terminus are different from one another, and
n is a natural number from 1 to 10, x is a natural number from 1 to 40, y is a natural number from 1 to 40, and x and y are different natural numbers, wherein the functional domain is a DNA cleaving domain, wherein the editing of a TCR gene is removal of an endogenous TCR gene, and wherein the DNA binding domain comprises the amino acid sequence of SEQ ID NO: 86, the amino acid sequence of SEQ ID NO: 87, the amino acid sequence of SEQ ID NO: 88, the amino acid sequence of SEQ ID NO: 89, the amino acid sequence of SEQ ID NO: 90, or the amino acid sequence of SEQ ID NO: 91.
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