CPC C12N 9/1252 (2013.01) [C12Q 1/6869 (2013.01); C12Y 207/07007 (2013.01)] | 22 Claims |
1. A composition comprising:
a plurality of engineered polymerases comprising an amino acid sequence that is at least 85% identical to SEQ ID NO:1 and has at least one amino acid substitution: Asp141Ala or Glu143Ala, and
a plurality of modified nucleotides comprising a removable chain terminating moiety at the 3′ carbon position of the sugar group.
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