CPC C12N 9/0069 (2013.01) [C12N 9/0071 (2013.01); C12Y 114/14003 (2013.01)] | 5 Claims |
1. A modified bacterial luciferase having improved activity as compared to a control bacterial luciferase, wherein the improved activity is an increase in light production and/or slower signal decay, wherein the LuxA subunit of the modified bacterial luciferase comprises an amino acid sequence that is at least 99% identical to the LuxA subunit of the control bacterial luciferase, wherein the LuxA subunit of the control bacterial luciferase has the amino acid sequence of SEQ ID NO:2, wherein:
(a) the amino acid of the modified bacterial luciferase corresponding to position 170 of SEQ ID NO:2 is R, W, or K;
(b) the amino acid of the modified bacterial luciferase corresponding to position 102 of SEQ ID NO:2 is K;
(c) the amino acid of the modified bacterial luciferase corresponding to position 22 of SEQ ID NO:2 is H; or
(d) the amino acid of the modified bacterial luciferase corresponding to position 166 of SEQ ID NO:2 is Y.
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