CPC C12N 5/0655 (2013.01) [A61P 9/00 (2018.01); C12N 5/0606 (2013.01); C12N 5/0647 (2013.01); C12N 5/0657 (2013.01); C12N 2501/04 (2013.01); C12N 2501/415 (2013.01); C12N 2501/70 (2013.01); C12N 2506/45 (2013.01)] | 6 Claims |
1. A method for producing a cardiomyocyte, wherein said method comprises (1) culturing human pluripotent stem cells on plates coated with a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma and in human pluripotent stem cell culture medium for 2 days, (2) after the 2 days, supplementing the medium of (1) with 12 μM CHIR plus 10 nM rapamycin and wherein the cells are cultured for 3 days, (3) after the 3 days, transferring and then culturing the cells of (2) in a medium comprising RPMI/B27 minus insulin supplemented with 12 μM CHIR plus 10 nM rapamycin without insulin for 1 day, and (4) after the 1 day, supplementing the medium of (3) with XAV939 plus 10 μM KY02111 for 4 days without medium replacement.
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