US 11,788,057 B2
3D label-free contactless formation of cellular structures and co-cultures through diamagnetophoresis
Abdel Rahman Abdel Fattah, Etobicoke (CA); Rakesh Prasad Sahu, Hamilton (CA); Fei Geng, Hamilton (CA); Sarah Mishriki, Scarborough (CA); Elvira Meleca, Hamilton (CA); Ishwar K. Puri, Ancaster (CA); and Suvojit Ghosh, Hamilton (CA)
Assigned to McMaster University, Hamilton (CA)
Filed by McMaster University, Hamilton (CA)
Filed on Nov. 1, 2018, as Appl. No. 16/178,172.
Claims priority of provisional application 62/580,088, filed on Nov. 1, 2017.
Prior Publication US 2019/0127685 A1, May 2, 2019
Int. Cl. C12N 5/00 (2006.01); G01N 33/50 (2006.01); C12Q 1/6876 (2018.01)
CPC C12N 5/0062 (2013.01) [C12Q 1/6876 (2013.01); G01N 33/5032 (2013.01); G01N 33/5082 (2013.01); C12N 2513/00 (2013.01); C12N 2527/00 (2013.01); C12Q 2600/136 (2013.01)] 22 Claims
 
1. A method of forming an assembly of particles in a plurality of receptacles, the method comprising:
combining a plurality of diamagnetic particles with a paramagnetic agent to form a suspension of particles in paramagnetic solutions in the plurality of receptacles, the diamagnetic particles including cells; and
applying external magnetic fields to the suspension of particles in the paramagnetic solutions to form one or more regions of lower magnetic field strength, the external magnetic fields being provided by aligning an array of magnet arrangements with the plurality of receptacles,
wherein the diamagnetic particles in the suspensions move towards the one or more regions of lower magnetic field strength forming the assembly of particles.