US 11,788,047 B2
Parallel bioreactor system
Shun Luo, Huzhou (CN); Fulin Hu, Huzhou (CN); Shiping Yuan, Huzhou (CN); and Jinpei Song, Huzhou (CN)
Assigned to Zhejiang Jinyishengshi Bioengineering Co., Ltd, Huzhou (CN)
Filed by Zhejiang Jinyishengshi Bioengineering Co., Ltd, Zhejiang (CN)
Filed on Jul. 20, 2021, as Appl. No. 17/381,146.
Application 17/381,146 is a continuation of application No. 15/391,927, filed on Dec. 28, 2016, granted, now 11,136,541.
Prior Publication US 2021/0348100 A1, Nov. 11, 2021
Int. Cl. C12M 1/00 (2006.01); C12M 3/06 (2006.01); C12M 1/36 (2006.01); C12M 1/34 (2006.01); C12M 3/00 (2006.01)
CPC C12M 23/58 (2013.01) [C12M 23/14 (2013.01); C12M 23/28 (2013.01); C12M 23/38 (2013.01); C12M 23/48 (2013.01); C12M 27/16 (2013.01); C12M 29/10 (2013.01); C12M 41/12 (2013.01); C12M 41/26 (2013.01); C12M 41/32 (2013.01); C12M 41/42 (2013.01); C12M 41/44 (2013.01); C12M 41/48 (2013.01)] 13 Claims
OG exemplary drawing
 
1. A culture method for culturing cells and/or microorganisms comprising:
providing a parallel bioreactor system comprising:
an oscillator for generating oscillating motion;
a plurality of culture vessels mounted on the oscillator, wherein each culture vessel is provided with an inner cavity;
disposable culture bags arranged in the inner cavities of the culture vessels and used for accommodating a culture solution;
a support and a shaking plate, wherein the shaking plate generates the oscillating motion relative to the support, and the culture vessels are mounted on the same shaking plate; and
wherein the oscillator consists of a motor, a main transmission eccentric shaft and four supporting eccentric shafts, the main transmission eccentric shaft and the four supporting eccentric shafts are connected between the support and the shaking plate by bearings, the motor drives the main transmission eccentric shaft and thus drives the shaking plate to carry out eccentric rotation about a vertical rotating center in the horizontal oscillating motion according to a set amplitude;
a control system, wherein the control system controls the oscillating motion of the oscillator and parameters of the culture solution in each of the disposable culture bags, wherein the control system includes a master control console and a plurality of reaction controllers, the master control console controls the motion of the oscillator and each reaction controller is connected to the corresponding culture vessel to receive the parameters from each of the disposable culture bags and carry out related operations on the culture vessel; and
independently monitoring and controlling each culture bags through the control system,
wherein each of the four supporting eccentric shafts is evenly distributed on the bottom of the oscillator, a balancing weight is mounted on each supporting eccentric shaft, and the balancing weight forms an angle of 180° with the eccentric direction to balance a centrifugal force generated by a load in an oscillating process of the oscillator.