	US 11,787,877 B2
	Antibody with non-natural amino acid introduced therein
Kensaku Sakamoto, Saitama (JP); Kazumasa Ohtake, Saitama (JP); Yoshimi Yamaguchi, Saitama (JP); Shigeyuki Yokoyama, Saitama (JP); Tatsuo Yanagisawa, Saitama (JP); Akiko Matsumoto, Saitama (JP); Akifumi Kato, Tokyo (JP); Yasuhisa Shiraishi, Tokyo (JP); Kaname Kimura, Tokyo (JP); and Masakazu Homma, Tokyo (JP)
Assigned to RIKEN, Saitama (JP); and KYOWA KIRIN CO., LTD., Tokyo (JP)
Filed by RIKEN, Wako (JP); and KYOWA KIRIN CO., LTD., Tokyo (JP)
Filed on Dec. 22, 2020, as Appl. No. 17/131,135.
Application 17/131,135 is a division of application No. 15/753,724, granted, now 10,906,990, previously published as PCT/JP2016/074056, filed on Aug. 18, 2016.
Claims priority of application No. 2015-162160 (JP), filed on Aug. 19, 2015.
Prior Publication US 2021/0107997 A1, Apr. 15, 2021
Int. Cl. C07K 16/46 (2006.01); C07K 16/28 (2006.01); C07K 16/32 (2006.01); A61K 39/395 (2006.01); C07K 16/00 (2006.01); C07K 16/24 (2006.01); A61K 47/68 (2017.01); C07K 16/22 (2006.01); A61K 47/60 (2017.01)

CPC C07K 16/467 (2013.01) [A61K 39/395 (2013.01); A61K 47/60 (2017.08); A61K 47/6803 (2017.08); A61K 47/6849 (2017.08); A61K 47/6855 (2017.08); A61K 47/6875 (2017.08); A61K 47/6889 (2017.08); C07K 16/00 (2013.01); C07K 16/22 (2013.01); C07K 16/241 (2013.01); C07K 16/28 (2013.01); C07K 16/2863 (2013.01); C07K 16/2887 (2013.01); C07K 16/32 (2013.01); C07K 16/46 (2013.01); C07K 2317/14 (2013.01); C07K 2317/20 (2013.01); C07K 2317/21 (2013.01); C07K 2317/24 (2013.01); C07K 2317/33 (2013.01); C07K 2317/52 (2013.01); C07K 2317/522 (2013.01); C07K 2317/55 (2013.01); C07K 2317/71 (2013.01); C07K 2317/72 (2013.01); C07K 2317/732 (2013.01); C07K 2317/734 (2013.01); C07K 2319/30 (2013.01)]

14 Claims

1. A polynucleotide comprising a nucleotide sequence encoding a monoclonal IgG antibody or antigen-binding fragment thereof which comprises at least one lysine derivative,

at positions selected from the group consisting of 131, 177, and 199 of a heavy chain of a human IgG antibody and at positions 155, 191, and 197 of a κ chain of a human antibody numbering according to the EU-index,

wherein said lysine derivative is encoded by a nonsense codon, and

wherein said lysine derivative is selected from the group consisting of a Z-lysine derivative, N6-(((trans-cyclooct-2-en-1-yl)oxy)carbonyl)-L-lysine (hereinafter, abbreviated as “TCO*-Lys”), and N6-((bicyclo[6.1.0]non-4-yn-9-ylmethoxy)carbonyl)-L-lysine (hereinafter, abbreviated as “BCN-Lys”).