	US 11,787,873 B2
	Bispecific HER2 antibodies and methods of use
Rebecca Croasdale-Wood, Penzberg (DE); Lydia Jasmin Hanisch, Birmensdorf (CH); Guy Georges, Habach (DE); Thomas Hofer, Zurich (CH); Ralf Hosse, Mettmenstetten (CH); Christian Klein, Bonstetten (CH); Ekkehard Moessner, Kreuzlingen (CH); Samuel Moser, Rotkreuz (CH); Wolfgang Schaefer, Mannheim (DE); Juergen Michael Schanzer, Munich (DE); Werner Scheuer, Penzberg (DE); Claudio Sustmann, Munich (DE); and Pablo Umana, Wollerau (CH)
Assigned to Hoffmann-La Roche Inc., Little Falls, NJ (US)
Filed by Hoffmann-La Roche Inc., Little Falls, NJ (US)
Filed on Jan. 16, 2020, as Appl. No. 16/744,697.
Application 16/744,697 is a division of application No. 15/186,098, filed on Jun. 17, 2016, granted, now 10,584,178.
Application 15/186,098 is a continuation of application No. PCT/EP2014/078375, filed on Dec. 18, 2014.
Prior Publication US 2020/0291131 A1, Sep. 17, 2020
Int. Cl. C07K 16/32 (2006.01); A61K 31/713 (2006.01); C12N 5/10 (2006.01); C12N 15/63 (2006.01); A61K 39/00 (2006.01)

CPC C07K 16/32 (2013.01) [A61K 31/713 (2013.01); C12N 5/10 (2013.01); C12N 15/63 (2013.01); A61K 2039/505 (2013.01); C07K 2317/24 (2013.01); C07K 2317/31 (2013.01); C07K 2317/35 (2013.01); C07K 2317/41 (2013.01); C07K 2317/51 (2013.01); C07K 2317/515 (2013.01); C07K 2317/52 (2013.01); C07K 2317/522 (2013.01); C07K 2317/55 (2013.01); C07K 2317/56 (2013.01); C07K 2317/565 (2013.01); C07K 2317/567 (2013.01); C07K 2317/622 (2013.01); C07K 2317/64 (2013.01); C07K 2317/66 (2013.01); C07K 2317/73 (2013.01); C07K 2317/732 (2013.01); C07K 2317/734 (2013.01); C07K 2317/92 (2013.01); C07K 2317/94 (2013.01)]

17 Claims

1. A bispecific antibody, comprising a first antigen binding site specific for extracellular domain II of HER2 and a second antigen binding site specific for extracellular domain IV of HER2, wherein the antibody comprises:

(a) a first heavy chain comprising a heavy chain CDR1 of SEQ ID NO: 58, a heavy chain CDR2 of SEQ ID NO: 15, and a heavy chain CDR3 of SEQ ID NO: 59, and

(b) a second heavy chain comprising a heavy chain CDR1 of SEQ ID NO: 20, a heavy chain CDR2 of SEQ ID NO: 29 and a heavy chain CDR3 of SEQ ID NO: 30

(c) a first light chain, comprising a light chain CDR1 of SEQ ID NO: 89, a light chain CDR2 of SEQ ID NO: 90, and a light chain CDR3 SEQ ID NO: 19, and

(d) a second light chain, comprising a light chain CDR1 of SEQ ID NO: 89, a light chain CDR2 of SEQ ID NO: 90, and a light chain CDR3 SEQ ID NO: 19.