	US 11,787,843 B2
	High productivity algal mutants having reduced photosynthetic antenna
Eric R. Moellering, San Diego, CA (US); Nicholas Bauman, San Diego, CA (US); Randor R. Radakovits, Poway, CA (US); Roberto Spreafico, San Diego, CA (US); Fedor Kuzminov, San Diego, CA (US); Imad Ajjawi, San Diego, CA (US); Saheed Imam, San Diego, CA (US); Andrew Schultz, San Diego, CA (US); Kathleen Kwok, San Diego, CA (US); Moena Aqui, San Diego, CA (US); Jennifer Nominati, San Diego, CA (US); John Verruto, San Diego, CA (US); and Shaun Bailey, San Diego, CA (US)
Assigned to Viridos, Inc., La Jolla, CA (US)
Filed by Synthetic Genomics, Inc., La Jolla, CA (US)
Filed on Feb. 17, 2021, as Appl. No. 17/178,179.
Application 17/178,179 is a division of application No. 15/859,094, filed on Dec. 29, 2017, granted, now 10,968,259.
Claims priority of provisional application 62/441,002, filed on Dec. 30, 2016.
Prior Publication US 2021/0188924 A1, Jun. 24, 2021
Int. Cl. C12N 1/12 (2006.01); C07K 14/405 (2006.01); C12N 1/36 (2006.01); C12N 15/82 (2006.01); C12P 21/00 (2006.01); C12N 15/01 (2006.01); C12P 7/6445 (2022.01); A01H 1/06 (2006.01); C12P 7/649 (2022.01); C12P 7/6454 (2022.01); C12R 1/89 (2006.01)

CPC C07K 14/405 (2013.01) [A01H 1/06 (2013.01); C12N 1/12 (2013.01); C12N 1/125 (2021.05); C12N 1/36 (2013.01); C12N 15/01 (2013.01); C12N 15/8269 (2013.01); C12P 7/649 (2013.01); C12P 7/6445 (2013.01); C12P 7/6454 (2013.01); C12P 21/00 (2013.01); C12R 2001/89 (2021.05); Y02E 50/10 (2013.01)]

19 Claims

1. A method of isolating a mutant Chlorophyte alga having higher biomass productivity with respect to the progenitor alga from which it is derived, comprising:

culturing a Chlorophyte algal strain under photoautotrophic semi-continuous or continuous conditions having a disruption in an SGI1 gene encoding a polypeptide having at least 90% sequence identity to SEQ ID NO: 3 or SEQ ID NO: 14-16; and

isolating at least one strain from the culture, wherein the at least one isolated strain has a higher biomass productivity than the progenitor alga;

thereby isolating a mutant Chlorophyte alga having higher biomass productivity with respect to the progenitor alga from which it is derived.