US 11,786,866 B2
Process control systems and methods for use with filters and filtration processes
Eva Gefroh, Newcastle, WA (US); Randolph W. Schweickart, Woodinville, WA (US); Krista Petty, Newbury Park, CA (US); Gregory Frank, Thousand Oaks, CA (US); Christine Salstrom Terpsma, Kenmore, WA (US); Arthur C. Hewig, III, Newbury Park, CA (US); and Joseph Edward Shultz, Binningen (CH)
Assigned to AMGEN INC., Thousand Oaks, CA (US)
Filed by AMGEN INC., Thousand Oaks, CA (US)
Filed on Jun. 29, 2021, as Appl. No. 17/362,282.
Application 16/743,728 is a division of application No. 15/302,762, granted, now 10,583,397, previously published as PCT/US2015/030599, filed on May 13, 2015.
Application 17/362,282 is a continuation of application No. 16/743,728, filed on Jan. 15, 2020, granted, now 11,077,404.
Claims priority of provisional application 61/992,595, filed on May 13, 2014.
Prior Publication US 2021/0339196 A1, Nov. 4, 2021
Int. Cl. B01D 61/22 (2006.01); B01D 61/14 (2006.01); B01D 61/18 (2006.01); C07K 1/34 (2006.01)
CPC B01D 61/22 (2013.01) [B01D 61/145 (2013.01); B01D 61/146 (2022.08); B01D 61/18 (2013.01); C07K 1/34 (2013.01); B01D 2311/04 (2013.01); B01D 2311/06 (2013.01); B01D 2313/18 (2013.01); B01D 2313/243 (2013.01); B01D 2315/10 (2013.01)] 32 Claims
OG exemplary drawing
 
1. A process control method for the concentration of microfiltration harvest fluid during a harvest process using single pass tangential flow with filtrate flow control, the process control method comprising:
separating cells and cell debris from a protein of interest in a harvest stream from a bioreactor with a microfiltration element disposed in-line with the harvest stream to create a microfiltration filtrate containing the protein of interest;
pumping the microfiltration filtrate containing the protein of interest through a single-pass tangential flow filter having an inlet, a permeate outlet and a retentate outlet; and
pumping permeate from the permeate outlet of the single-pass tangential flow filter to vary a flow reduction factor, where the flow reduction factor is a ratio of feed flow into the inlet of the single-pass tangential flow filter to retentate flow out of the retentate outlet of the single-pass tangential flow filter, wherein the flow reduction factor is varied to achieve a target volume reduction factor, wherein the volume reduction factor is the ratio of cumulative feed volume of the feed flow during the harvest process to cumulative retentate volume of the retentate flow during the harvest process.