CPC B01D 61/22 (2013.01) [B01D 61/145 (2013.01); B01D 61/146 (2022.08); B01D 61/18 (2013.01); C07K 1/34 (2013.01); B01D 2311/04 (2013.01); B01D 2311/06 (2013.01); B01D 2313/18 (2013.01); B01D 2313/243 (2013.01); B01D 2315/10 (2013.01)] | 32 Claims |
1. A process control method for the concentration of microfiltration harvest fluid during a harvest process using single pass tangential flow with filtrate flow control, the process control method comprising:
separating cells and cell debris from a protein of interest in a harvest stream from a bioreactor with a microfiltration element disposed in-line with the harvest stream to create a microfiltration filtrate containing the protein of interest;
pumping the microfiltration filtrate containing the protein of interest through a single-pass tangential flow filter having an inlet, a permeate outlet and a retentate outlet; and
pumping permeate from the permeate outlet of the single-pass tangential flow filter to vary a flow reduction factor, where the flow reduction factor is a ratio of feed flow into the inlet of the single-pass tangential flow filter to retentate flow out of the retentate outlet of the single-pass tangential flow filter, wherein the flow reduction factor is varied to achieve a target volume reduction factor, wherein the volume reduction factor is the ratio of cumulative feed volume of the feed flow during the harvest process to cumulative retentate volume of the retentate flow during the harvest process.
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