	US 11,786,637 B2
	Methods and compositions for preparing transplant tissue
Eric Semler, Morganville, NJ (US); Mark Spilker, Kilchberg (CH); Kevin Wu, Morganville, NJ (US); Yen-Chen Huang, East Brunswick, NJ (US); Evangelia Chnari, Scotch Plains, NJ (US); Jeffrey Cartmell, Freehold, NJ (US); Morris Jacobs, Newton, NJ (US); Alison Ling, Piscataway, NJ (US); and Moon Hae Sunwoo, Old Tappan, NJ (US)
Assigned to Musculoskeletal Transplant Foundation, Edison, NJ (US)
Filed by Musculoskeletal Transplant Foundation, Edison, NJ (US)
Filed on Mar. 1, 2021, as Appl. No. 17/188,183.
Application 17/188,183 is a division of application No. 15/680,012, filed on Aug. 17, 2017, granted, now 10,973,953.
Claims priority of provisional application 62/376,843, filed on Aug. 18, 2016.
Prior Publication US 2021/0178022 A1, Jun. 17, 2021
Int. Cl. A61L 27/38 (2006.01); A61L 27/36 (2006.01); A01N 1/02 (2006.01)

CPC A61L 27/3895 (2013.01) [A01N 1/0284 (2013.01); A61L 27/3604 (2013.01); A61L 27/3683 (2013.01); A61L 27/38 (2013.01); A61L 2430/02 (2013.01)]

6 Claims

1. A method for providing an artificially primed tissue graft, comprising:

obtaining a tissue containing viable cells from a donor, wherein the viable cells are endogenous to the tissue and remain resident in the tissue;

priming the viable cells with one or more stimuli comprising simulated hypoxia to produce the artificially primed tissue graft which comprises an increased amount of angiogenic growth factors compared to a non-primed tissue, wherein when used to treat a patient the artificially primed tissue graft provides a benefit compared to non-primed tissue; and

decellularizing the primed tissue graft prior to grafting.