US 11,786,547 B2
Methods and compositions for disrupting biofilm utilizing chitosan-derivative compounds
Shenda M. Baker, Upland, CA (US); William P. Wiesmann, Chevy Chase, MD (US); and Stacy Marie Townsend, Rancho Cucamonga, CA (US)
Assigned to SYNEDGEN, INC., Claremont, CA (US)
Filed by SYNEDGEN, INC., Claremont, CA (US)
Filed on Sep. 16, 2019, as Appl. No. 16/572,053.
Application 16/572,053 is a continuation of application No. 15/419,436, filed on Jan. 30, 2017, abandoned.
Application 15/419,436 is a continuation of application No. 13/411,147, filed on Mar. 2, 2012, abandoned.
Application 13/411,147 is a continuation of application No. PCT/US2010/047758, filed on Sep. 2, 2010.
Claims priority of provisional application 61/239,180, filed on Sep. 2, 2009.
Prior Publication US 2020/0009182 A1, Jan. 9, 2020
Int. Cl. A61K 31/722 (2006.01); A61K 9/00 (2006.01)
CPC A61K 31/722 (2013.01) [A61K 9/0014 (2013.01); A61K 9/0043 (2013.01); A61K 9/0053 (2013.01); Y02A 50/30 (2018.01)] 18 Claims
 
1. A method of reducing the viscosity of viscous sputum in the respiratory system of a human subject having cystic fibrosis, the method comprising:
administering to the subject by inhalation a therapeutically effective amount of a composition comprising a derivatized chitosan, wherein the derivatized chitosan comprises a chitosan of the following formula (I):

OG Complex Work Unit Chemistry
wherein:
n is an integer between 20 and 6000; and
each R1 is independently selected for each occurrence from hydrogen, acetyl, and a group of formula (II): wherein, formula (II) is selected from

OG Complex Work Unit Chemistry
wherein at least 25% of R1 substituents are H, at least 1% of R1 substituents are acetyl, and at least 4-30% of R1 substituents are a group of formula (II), the molecular weight of the derivatized chitosan is between 10,000 and 150,000 Da, and the polydispersity index (PDI) of the derivatized chitosan is between 1.5 and 2.5, wherein, upon administration, the composition disrupts a preformed biofilm in the respiratory system of the subject and reduces the viscosity of viscous sputum in the subject relative to sputum that has not been contacted with the composition.