US 11,785,913 B2
Mushroom line J14756-s3 and methods and uses therefor
Richard Kerrigan, Kittanning, PA (US); Mark Wach, Allison Park, PA (US); Michelle Schultz, New Bethlehem, PA (US); Mark G. Loftus, Oakmont, PA (US); Michael A. Kessler, Kittanning, PA (US); William P. Swanik, Petrolia, PA (US); Anne Rodier, Saumur (FR); Anica Amini, Saint-Pierre-des-Corps (FR); Sylvie Delbecque, Langeais (FR); and Richard Rucklidge, West Sussex (GB)
Assigned to SYLVAN AMERICA, INC., Kittanning, PA (US)
Appl. No. 16/465,214
Filed by SYLVAN AMERICA, INC., Kittanning, PA (US)
PCT Filed Nov. 28, 2017, PCT No. PCT/US2017/063405
§ 371(c)(1), (2) Date May 30, 2019,
PCT Pub. No. WO2018/102290, PCT Pub. Date Jun. 7, 2018.
Claims priority of provisional application 62/428,597, filed on Dec. 1, 2016.
Prior Publication US 2020/0084993 A1, Mar. 19, 2020
Int. Cl. A01H 15/00 (2006.01); C12N 1/14 (2006.01); A01G 18/00 (2018.01)
CPC A01H 15/00 (2013.01) [A01G 18/00 (2018.02); C12N 1/14 (2013.01); C12N 1/145 (2021.05)] 27 Claims
 
1. An Agaricus bisporus culture comprising:
at least one set of chromosomes of an Agaricus bisporus line J14756-s3, a culture of the line J14756-s3 having been deposited under the NRRL Accession Number 67317, wherein said chromosomes comprise all alleles reported for the line J14756-s3 at the sequence-characterized marker loci listed in at least one of Tables I and II, wherein Table I is provided as follows:
TABLE I
 
Scaffold Position of SNP Cultures:
[H97 V2.0 ref. coordinates] H97 Wild Line
 
1 99995 CTACATTGA CTACGTTGA
1 349966 AAGGTGGTT AAGGCGGTT
1 600059 TTTTTTTT- TTTTTTTTC
1 850014 CCTTTTCAC TCTTGTCAC
 
 
 TCTTGTCACTCTTGTCAC
1 1099971 GTCGACACC GTCGACACC
1 1350278 GGAGAGTCG GGAGAGTCG
1 1599956 AATAAGCGC AATAAGCGC
1 1850032 CGAGTAATT CGAGCAATT
1 2119049 ACAATCCAA ACAACTCAA
1 2400243 ACTTCATGA ACTTGATGA
1 2612870 AATAGGAGT AATAGGAGT
1 2858975 GCCGTTCTTGCCGCTCTTG
 
 
 CCGCTCTTGCCGCTCTT
1 2804522 GAAGACGAC GAAGGCGAC
1 3047987 AAGGGGGGG AAGGAGGGG
1 3212182 CACTACGTG CACTGCGTG
1 3256057 TATCTGTTT TATCCGTTT 
 
 
 TATCCGTTT TATCCGTTT
 
2 101820 ATTAAAGAT ATTAAAGAT
2 350156 TCGGGGGTG TCGGAGGTG
2 600112 ATGTATACG ATGTGTACG
2 850338 TGGTGCTAA TGGTTCTAA
 
 
 
 TGGTGCTAA
2 1099413 CCTGACTCA CCTGGCTCA
2 1349512 CTCAGCAGT CTCAACAGT
2 1600085 CACAATGCC CACATTGCC
2 1901773 ACTCGAATT ACTAAAATT
2 2150201 GTCGTAGGT GTCGAAGGT
2 2400281 TCAAAACCC TCAACACCC
2 2650136 ATAATTCCTATAAGTCCT
2 2903620 ACTAAAAGA ACTAAAAGA
2 3048019 GTCCGCTGC GTCCGCTGC
 
3 57117 TATAGCAGT TATGACAGT
3 65650 GGCGCTTTTGGCGGTTTTG
 
 
 GCGGTTTTGGCGGTTTT
3 119181 TTTATACTCTTTATACTCT
 
 
 TTATACTCTTTATACTC
3 250112 GCAGGAGAG GCCGAAGAG
3 750000 GTCCGGCCA GTCCGGCCA
3 750074 AGTTTTTTC AGTTATTTC  
 
 
 AGTTTTTTC AGTTwTTTC
3 1250000 TTTTTCCGG TTTTTCCGG  
 
 
 TTTTTCCGG TTTTTCCGG
3 1250161 AGTCTCCTT AGTCCCCTT  
 
 
 AGTCTCCTT AGTCyCCTT
3 1750000 ACGCCTGAC ACGCCTGAC
3 1750033 TTTTTTGCT TTTTGTGCT  
 
 
 TTTTTTGCT TTTTkTGCT
3 2250000 CGTGGCGAT CGTGGCGAT
3 2250027 TGTATCAAG TGTGCCAAG
3 2520748 TAATTCCAC TAATGCCAC
 
4 100004 GAGTGATAA GAGTGATAA
4 490648 CGATCGCGT CGATTGCGT
4 598147 GATCGACAG GATCAACAG
4 852119 CGAATATTC CGAATATTC
4 1100085 GATGCCGAA GATGACGAA
4 1350536 CGAACTCGG CGAAATCGG
4 1599885 GATACTTGC GATACTTGC
4 1850288 ATTCGTGTAATTCATGTAA
 
 
 TTCGTGTAATTCrTGTA
4 2100356 TCAGAGACC TCAGAGACC
4 2284257 TCTGGACTG TCTGAACTG
 
5 100211 TCCTTGAATTCCTGGAAT
5 350872 GGCGTGCCC GGCGTGCCC
5 599922 CGTCATTCA CGTCATTCA
5 851262 TAATTCTCTTAATCGTCTT
 
 
 AATCGTCTTAATCGTCT
5 1099776 ACATTGACA ACATCGACA
5 1352539 TTGTGATCCTTGTGATCCT
 
 
 TGTTGTCCTTGTkrTCC
5 1599904 AACTTCCTT AACTCCCTT  
 
 
 AACTCCCTT AACTCCCTT
5 1851458 AAATAATCC AAATTCTCC
5 2100025 CCCTTAGTCCCCTTAGTCC
 
 
 CCTCAGTC
5 2278878 GGTCAAAAA GGTCAAAAA
 
6 106294 GCCATCTCG GCCACCTCG
6 350337 CATTTGGTTCATTCGGTTC
 
 
 ATTTGGTTCATTyGGTT
6 600047 GGAGCATTT GGAGTATTT
6 849985 AGTTCAGGA AGTTCAGGA
6 1098535 CAAAGATTG CAAAAATTG
6 1349453 TGTCGGTAG TGTCAATAG
6 1600000 AAACCTGGA AAACCTGGA
6 1764645 AACCAGATT AACCGGATT
6 2000087 GATTTTGCGGATTTTGCGG
 
 
 ATTTTGCGGATTTTGCG
6 2252662 GGGTTGGTA GGGTCGGTA
 
7 100284 GAAATTCAG GAAATTCAG
7 350044 ATATTCTTT ATATCCTTT  
 
 
 ATATTCTTT ATATyCTTT
7 600111 CAATTATTACAATCATTA
7 850246 TGACGCATA TGACGCATA
7 1100248 TCACGGAAG TCACGGAAG
7 1350089 CTTTTCCCC CTTTTCCCCC  
 
 
 TTTTCCCC CTTTTCCCC
7 1605047 ATACTTGGC ATACTTGAC
7 1860993 GTCAACCGG GTCATCCGG
7 1898793 TCCGCATAA TCCGCATAA
7 1991505 TCTACGGTTTCTACGGTTT
 
 
 CTACGGTTTCTACGGTT
 
8 350000 ATTGACGCG ATTGGCGCG
8 610549 GAACTTGAT GAATACGAT
8 1100000 CATACGATC CATACGATC
8 1100546 GCCCCAGAA GCCCCAGAA
8 1350000 AGCTTAACA AGCTTAACA
8 1350240 ACGGGTACT ACGGATACT
8 1600100 CTGAACCCT CTGAACCCT
 
9 100105 CTCAACCGA CTCAACCGA
9 352455 AGTCCTCCA AGTCCTCCA
9 599950 TGGTATCCC TGGTGTCCC
9 1010845 GGGTGGTGA GGGTGGTGA
9 1244202 GATGAAGAT GATGAAGAT
9 1504476 TACTGTACC TACTGTACC
9 1656962 TATCTACTGTATCTACTGT
 
 
 ATCTACTGTATCTACTG
 
10 100438 AATTAATTTGATTAATTTA
 
 
 ATTAATTTrATTAATTT
10 350030 GCGGTTCAA GCGGTTCAA
10 600032 TTACACTGG TTACACTGG
10 850000 TCGGTCGGA TCGGTCGGA
10 860249 CCGCAAATT CCGCGAATT
10 1173178 ATCCCCAAT ATCCTCAAT
10 1303902 TGATTTACTTGATTTACTT
 
 
 GATTTACTTGATTTACT
10 1490452 AATCAGATG AATCAGATG
 
11 101855 CCAGCCTGT CCAGTCTGT
11 350000 GTCAGCAAG GTCAGCAAG
11 350025 GATAAAACA GATAAAACA
11 600000 ATGGGCGCG ATGGGAGCG
11 929659 GGAATATCA GGAAGTTCA
11 1100608 AGTGGTCTTAGTGGTCTTA
 
 
 GTGATCTTAGTGrTCTT
11 1240230 ACAAGTTTC ACAAGTTTC
 
12 100000 CCTTCTAGTCCTTCTAGTC
 
 
 CTTCTAGTCCTTCTAGT
12 109790 GTCTGCACC GTCTACACC
12 1000000 CGAGGAGGA CGAGGAGGA
 
13 100697 ACGTCTTTAACGTCTTTAA
 
 
 CGTCTTTAACGTCTTTA
13 370521 TTTGAGTCATTTGTGTCAT
 
 
 TTGTGTCATTTGTGTCA
13 604345 CTTCAGCAT CTTCAGCAT
13 850249 GGCTAGTAA GGCTAGTAA
 
14 113109 AGGGAAATA AGGGGAATA
14 372086 CGATCCCTTCGATTCCTTC
 
 
 GATCCCTTCGATyCCTT
14 725684 ATGAGTTCG ATGAGTTTG
 
15 150013 GTGGCCCGT GTGGACCGT
15 449860 GAATTTCGG GAATTTCGG
 
16 205778 CAAGGTCTG CAAGATCTG
16 400000 CCTCGGATT CCTCGGATT
16 403998 CCAAGTACG CCAAATACG
 
17 120000 TATTCTTCA TATTCTTCA  
 
 
 TATTCTTCA TATTCTTCA
17 134676 TTGCCCAGC TTGCTCAGC
17 338415 TGAGAAGCC TGAGAAGCC
17 449833 ATCAAACAA ATCAAACAA
 
18 101884 ATTACGGAC ATTACGGAC
18 112940 GCGGGTGGG GCGGCTGGG
 
19 98520 GCTATTGGG GCTATTGGG
19 98782 AAAATTGTT AAAAGTGTT
 
Scaffold Position of SNP Cultures:
[H97 V2.0 ref. coordinates] J14756-s3 J15051
 
1 99995 CTACGTTGA CTACGTTGA
1 349966 AAGGCGGTT AAGGCGGTT
1 600059 TTTTCTTT- TTTTyTTTC
1 850014
 
1 1099971 GTCGGCACC GTCGrCACC
1 1350278 GGAGGTTCG GGAGrkTCG
1 1599956 AATAGGCGC AATArGCGC
1 1850032 CGAGCAATT CGAGCAATT
1 2119049 ACAACTCAA ACAACTCAA
1 2400243 ACTTGATGA ACTTGATGA
1 2612870 AATAAGAGT AATArGAGT
1 2858975
 
1 2804522 GAAGGCGAC GAAGGCGAC
1 3047987 AAGGGGGGG AAGGrGGGG
1 3212182 CACTACGTG CACTrCGTG
1 3256057
 
 
2 101820 ATTAAAGAT ATTAAAGAT
2 350156 TCGGGGGTG TCGGrGGTG
2 600112 ATGTATACG ATGTrTACG
2 850338
 
 TGGTkCTAA
2 1099413 CCTGACTCA CCTGrCTCA
2 1349512 CTCAGCAGT CTCArCAGT
2 1600085 CACAATGCC CACAwTGCC
2 1901773 ACTCAAATT ACTmAAATT
2 2150201 GTCGTAGGT GTCGwAGGT
2 2400281 TCAACACCC TCAACACCC
2 2650136 ATAATTCCT ATAAkTCCT
2 2903620 ACTAAAAGA ACTAAAAGA
2 3048019 GTCCGCTGC GTCCGCTGC
 
3 57117 TATGACAGT TATGACAGT
3 65650
 
3 119181
 
3 250112 GCAGGAGAG GCmGrAGAG
3 750000 GTCCGGCCA GTCCGGCCA
3 750074
 
3 1250000
 
3 1250161
 
3 1750000 ACGCCTGAC ACGCCTGAC
3 1750033
 
3 2250000 CGTGGCGAT CGTGGCGAT
3 2250027 TGTGCCAAG TGTryCAAG
3 2520748 TAATTCCAC TAATkCCAC
 
4 100004 GAGTGATAA GAGTGATAA
4 490648 CGATCGCGT CGATyGCGT
4 598147 GATCGACAG GATCrACAG
4 852119 CGAATATTC CGAATATTC
4 1100085 GATGCCGAA GATGmCGAA
4 1350536 CGAACTCGG CGAAmTCGG
4 1599885 GATACTTGC GATACTTGC
4 1850288
 
4 2100356 TCAGGGACC TCAGrGACC
4 2284257 TCTGAACTG TCTGrACTG
 
5 100211 TCCTGGAAT TCCTGGAAT
5 350872 GGCGCGCCC GGCGyGCCC
5 599922 CGTCGTTCA CGTCrTTCA
5 851262
 
5 1099776 ACATTGACA ACATyGACA
5 1352539
 
5 1599904
 
5 1851458 AAATTCTCC AAATTCTCC
5 2100025
 
 CCCTyAGTC
5 2278878 AGTCAAAAA rGTCAAAAA
 
6 106294 GCCATCTCG GCCAyCTCG
6 350337
 
6 600047 GGAGCATTT GGAGyATTT
6 849985 AGTTCAGGA AGTTCAGGA
6 1098535 CAAAGATTG CAAArATTG
6 1349453 TGTCGGTAG TGTCrrTAG
6 1600000 AAACCTGGA AAACCTGGA
6 1764645 AACCGGATT AACCrGATT
6 2000087
 
6 2252662 GGGTTGGTA GGGTyGGTA
 
7 100284 GAAACTCAG GAAAyTCAG
7 350044
 
7 600111 CAATTATTA CAATyATTA
7 850246 TGACGCATA TGACGCATA
7 1100248 TCACGGAAG TCACrGAAG
7 1350089
 
7 1605047 ATACTTGGC ATACTTGrC
7 1860993 GTCAACCGG GTCAwCCGG
7 1898793 TCCGCATAA TCCGCATAA
7 1991505
 
 
8 350000 ATTGGCGCG ATTGGCGCG
8 610549 GAATTTGAT GATwyGAT
8 1100000 CATACGATC CATACGATC
8 1100546 GCCCGAGAA GCCCsAGAA
8 1350000 AGCTTAACA AGCTTAACA
8 1350240 ACGGGTACT ACGGrTACT
8 1600100 CTGAACCCT CTGAACCCT
 
9 100105 CTCAGCCGA CTCArCCGA
9 352455 AGTCTCCCA AGTCyyCCA
9 599950 TGGTGTCCC TGGTGTCCC
9 1010845 GGGTAGTGA GGGTrGTGA
9 1244202 GATGGAGAT GATGrAGAT
9 1504476 TACTATACC TACTrTACC
9 1656962
 
 
10 100438
 
10 350030 GCGGTTCAA GCGGTTCAA
10 600032 TTACACTGG TTACACTGG
10 850000 TCGGTCGGA TCGGTCGGA
10 860249 CCGCAAATT CCGCrAATT
10 1173178 ATCCTCAAT ATCCTCAAT
10 1303902
 
10 1490452 AATCAGATG AATCAGATG
 
11 101855 CCAGCCTGT CCAGyCTGT
11 350000 GTCAGCAAG GTCAGCAAG
11 350025 GATATAACA GATAwAACA
11 600000 ATGGGAGCG ATGGGrGCG
11 929659 GGAATATCA GGAAkwTCA
11 1100608
 
11 1240230 ACAAATTTC ACAArTTTC
 
12 100000
 
12 109790 GTCTGCACC GTCTrCACC
12 1000000 CGAGAAGGA CGAGrAGGA
 
13 100697
 
13 370521
 
13 604345 CTTCAGCAT CTTCAGCAT
13 850249 GGCTAGTAA GGCTAGTAA
 
14 113109 AGGGAAATA AGGGrAATA
14 372086
 
14 725684 ATGAATTCG ATGArTTyG
 
15 150013 GTGGCCCGT GTGGmCCGT
15 449860 GAATTTCGG GAATTTCGG
 
16 205778 CAAGATCTG CAAGATCTG
16 400000 CCTCGGATT CCTCGGATT
16 403998 CCAAGTACG CCAArTACG
 
17 120000
 
 
17 134676 TTGCCCAGC TTGCyCAGC
17 338415 TGAGAAGCC TGAGAAGCC
17 449833 ATCAAACAA ATCAAACAA
 
18 101884 ATTACGGAC ATTACGGAC
18 112940 GCGGGTGGG GCGGsTGGG
 
19 98520 GCTATTGGG GCTATTGGG
19 98782 AAAATTGTT AAAAkTGTT
 
and wherein Table II is provided as follows:
TABLE II
 
 
 Markers:
 
 
 p1n150/ MFPC-
 
 
 
 
 Match
Strain: Mat 1-ELF FF AN AS ITS %
 
J15051  2/5 E3/E4 FF2/ N3/ SA/ I1/  [100%]
 
 
 
 FF2 N4 SD I5
 
 
B14528  2/5 E3/E4 FF1/ N3/ SC/ I1/ 66.7%
 
 
 
 FF3 N4 SD I2
 
 
28c, [Heirloom] 1T/5 E3/E4 FF1/ N2/ SA/ I1/ 58.3%
 
 
 
 FF3 N3 SD I1
 
 
28b, [Braun] 1T/5 E3/E4 FF1/ N2/ SB/ I1/ 50.0%
 
 
 
 FF4 N3 SD I1
 
 
PTA-6877 1T/2 E2/E3 FF1/ N2/ SD/ I1/ 33.3%
 
 
 
 FF1 N4 SD I2
 
 
S600 1T/2 E1/E3 FF2/ N2/ SC/ I1/ 41.7%
 
 
 
 FF2 N4 SD I2
 
 
J10263 1T/2 E1/E3 FF2/ N4/ SC/ I1/ 50.0%
 
 
 
 FF7 N7 SD I2
 
 
OFBlineage group 1T/3 E3/E6 FF1/ N4/ SC/ I1/ 41.7%
 
 
 
 FF2 N4 SD I3
 
 
J14756-s3 2 E3 FF2 N4 SD I5
 
 
wild line 5 E4 FF2 N3 SA I1.