US 11,754,823 B2
Cryo-light microscope and immersion medium for cryo-light microscopy
Thomas P. Burg, Goettingen (DE); Margherita Bassu, Goettingen (DE); Yara Mejia, Berkeley, CA (US); and Raffaele Faoro, Goettingen (DE)
Assigned to MAX-PLANCK-GESELLSCHAFT ZUR FÖRDERUNG DER WISSENSCHAFTEN E.V., Munich (DE)
Filed by Max-Planck-Gesellschaft zur Foerderung der Wissenschaften e.V., Munich (DE)
Filed on Mar. 18, 2020, as Appl. No. 16/822,173.
Application 16/822,173 is a continuation of application No. PCT/EP2018/075333, filed on Sep. 19, 2018.
Claims priority of application No. 17192011 (EP), filed on Sep. 20, 2017.
Prior Publication US 2020/0285036 A1, Sep. 10, 2020
Int. Cl. G02B 21/33 (2006.01); C07C 43/12 (2006.01); C09K 5/10 (2006.01); C07C 43/02 (2006.01)
CPC G02B 21/33 (2013.01) [C07C 43/02 (2013.01); C07C 43/12 (2013.01); C09K 5/10 (2013.01)] 9 Claims
OG exemplary drawing
 
1. A method of imaging a biological sample by light microscopy using an immersion objective, the method comprising imaging the biological sample by light microscopy,
wherein cryogenic liquid consisting essentially of a hydrofluoroether is arranged as an immersion medium between the biological sample to be imaged and the immersion objection, wherein the immersion objective is immersed in the immersion medium.