CPC G02B 21/33 (2013.01) [C07C 43/02 (2013.01); C07C 43/12 (2013.01); C09K 5/10 (2013.01)] | 9 Claims |
1. A method of imaging a biological sample by light microscopy using an immersion objective, the method comprising imaging the biological sample by light microscopy,
wherein cryogenic liquid consisting essentially of a hydrofluoroether is arranged as an immersion medium between the biological sample to be imaged and the immersion objection, wherein the immersion objective is immersed in the immersion medium.
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