CPC G01N 33/54386 (2013.01) [C12Q 1/68 (2013.01); C12Q 1/682 (2013.01); C12Q 1/6804 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/6816 (2013.01); C12Q 1/6818 (2013.01); C12Q 1/6823 (2013.01); C12Q 1/6832 (2013.01); C12Q 1/6874 (2013.01); G01N 33/543 (2013.01); G01N 33/54306 (2013.01); G01N 33/54333 (2013.01); G01N 33/58 (2013.01); G01N 33/587 (2013.01)] | 15 Claims |
1. A method for detecting two or more targets, comprising:
(1) contacting a sample being tested for the presence of two or more targets with two or more target-specific binding partners, wherein each target-specific binding partner is linked to a docking strand and wherein target-specific binding partners of different specificity are linked to different docking strands;
(2) optionally removing unbound target-specific binding partners;
(3) contacting the sample with enzyme-labeled strands capable of binding a docking strand, wherein the enzyme-labeled strands comprise horseradish peroxidase (HRP)-labeled strands;
(4) optionally removing unbound enzyme-labeled strands;
(5) contacting the sample with a substrate for the enzyme comprising a tyramide moiety linked to a fluorophore;
(6) allowing an enzymatic reaction to produce an amplification product;
(7) quenching the enzymatic reaction by removing the bound enzyme-labeled strands;
(8) repeating steps (3)-(7),
(9) after step (8), imaging the sample to detect the presence or absence of two or more targets; and
(10) optionally repeating at least some of steps (1)-(9).
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