US 11,754,559 B2
Methods of assaying proteins
Parag Mallick, San Mateo, CA (US)
Assigned to NAUTILUS SUBSIDIARY, INC., Seattle, WA (US)
Filed by NAUTILUS SUBSIDIARY, INC., Seattle, WA (US)
Filed on Jan. 20, 2021, as Appl. No. 17/153,877.
Application 17/153,877 is a continuation of application No. 16/659,132, filed on Oct. 21, 2019, granted, now 10,948,488.
Application 16/659,132 is a continuation of application No. 16/426,917, filed on May 30, 2019, granted, now 10,473,654, issued on Nov. 12, 2019.
Application 16/426,917 is a continuation of application No. PCT/US2017/064322, filed on Dec. 1, 2017.
Claims priority of provisional application 62/500,455, filed on May 2, 2017.
Claims priority of provisional application 62/429,063, filed on Dec. 1, 2016.
Prior Publication US 2021/0278400 A1, Sep. 9, 2021
Int. Cl. G01N 33/543 (2006.01); C12Q 1/6804 (2018.01)
CPC G01N 33/54353 (2013.01) [C12Q 1/6804 (2013.01); G01N 2458/10 (2013.01)] 23 Claims
 
1. A method for identifying a protein of a plurality of proteins, comprising:
(a) attaching a plurality of proteins to an array, wherein a protein of the plurality of proteins is immobilized at a position of the array;
(b) treating the plurality of proteins to remove posttranslational protein modifications;
(c) exposing the array to a plurality of different affinity reagents, wherein affinity reagents of the plurality of different affinity reagents have a known degree of binding non-specificity for proteins of the plurality of different proteins;
(d) determining a binding pattern of the affinity reagents bound to the protein; and
(e) identifying the protein using the known degree of binding non-specificity of the affinity reagents and the binding pattern of the affinity reagents bound to the protein.