US 11,754,553 B2
Agricultural endophyte-plant compositions, and methods of use
Geoffrey Von Maltzahn, Boston, MA (US); Richard Bailey Flavell, Thousand Oaks, CA (US); Gerardo V. Toledo, Belmont, MA (US); Allison Jack, Arlington, MA (US); David Morris Johnston, Cambridge, MA (US); Slavica Djonovic, Malden, MA (US); Luis Miguel Marquez, Belmont, MA (US); Yves Alain Millet, Newtonville, MA (US); Craig Sadowski, Somerville, MA (US); Jeffrey Lyford, Hollis, NH (US); and Alexander Naydich, Cambridge, MA (US)
Assigned to Indigo Ag, Inc., Boston, MA (US)
Filed by Indigo Ag, Inc., Boston, MA (US)
Filed on Dec. 30, 2020, as Appl. No. 17/138,109.
Application 17/138,109 is a continuation of application No. 16/151,131, filed on Oct. 3, 2018, granted, now 10,912,303.
Application 16/151,131 is a continuation of application No. 14/916,514, granted, now 10,136,646, issued on Nov. 27, 2018, previously published as PCT/US2014/054160, filed on Sep. 4, 2014.
Application 14/916,514 is a continuation in part of application No. 14/316,469, filed on Jun. 26, 2014, granted, now 9,113,636, issued on Aug. 25, 2015.
Claims priority of provisional application 61/959,861, filed on Sep. 4, 2013.
Claims priority of provisional application 61/959,870, filed on Sep. 4, 2013.
Claims priority of provisional application 61/959,871, filed on Sep. 4, 2013.
Claims priority of provisional application 61/959,875, filed on Sep. 4, 2013.
Claims priority of application No. PCT/US2014/044427 (WO), filed on Jun. 26, 2014.
Prior Publication US 2021/0372997 A1, Dec. 2, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/50 (2006.01); A01N 63/20 (2020.01); A01C 1/06 (2006.01); A01C 1/00 (2006.01)
CPC G01N 33/5097 (2013.01) [A01C 1/06 (2013.01); A01N 63/20 (2020.01); A01C 1/00 (2013.01)] 22 Claims
 
1. A method for preparing an agricultural seed preparation, comprising:
a) manually or mechanically inoculating a plurality of Glycine agricultural plant seeds with a formulation comprising a purified bacterial population at a concentration of at least 104 CFU/ml in a liquid formulation or at least 104 CFU/gm in a non-liquid formulation, the bacterial population comprising a Ochrobactrum bacterial endophyte comprising a 16S nucleic acid sequence comprising SEQ ID NO: 710 and exhibiting at least one of production of an auxin or production of acetoin, wherein the inoculated seeds and/or the Glycine agricultural plant grown therefrom comprises an increased production of a polypeptide of Aspartate aminotransferase glyoxysomal isozyme AAT1 precursor, wherein the Ochrobactrum bacterial endophyte is present in the formulation in an amount capable of providing a benefit to the inoculated Glycine plant seeds or to the Glycine agricultural plants grown from the inoculated Glycine plant seeds compared to reference Glycine seeds or reference Glycine agricultural plants, wherein the formulation further comprises a stabilizer, a fungicide, a preservative, or a combination thereof; and
b) packaging the inoculated seeds in a container.