CPC G01N 30/72 (2013.01) [C12M 41/30 (2013.01); G01N 30/7233 (2013.01); G01N 30/8665 (2013.01); G01N 2030/027 (2013.01); G01N 2030/045 (2013.01)] | 30 Claims |
1. A method for high-throughput quantitation of biomolecules, comprising:
(a) combining one or more calibrators with a mixed sample comprising one or more target analytes;
(b) separating the mixed sample using a chromatographic column;
(c) obtaining raw mass spectrometry (MS) data of the separated sample from (b); and
(d) processing the raw MS data using a machine learning model means to directly determine, in less than 12 minutes of total runtime measured from a beginning of the separation in (b), an absolute concentration of the one or more target analytes in the mixed sample.
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