US 11,754,536 B2
Methods and compositions for analyte quantification
Timothy Kassis, Saugus, MA (US); Jefferson Pruyne, Somerville, MA (US); Mark D. Simon, Somerville, MA (US); Mimoun Cadosch Delmar Akerman, Brookline, MA (US); Jennifer Campbell, Arlington, MA (US); Ana Henriques Da Costa, Cambridge, MA (US); Laura Kolinsky, Boston, MA (US); and John M. Geremia, Wayland, MA (US)
Assigned to MATTERWORKS INC, Somerville, MA (US)
Filed by MATTERWORKS INC, Somerville, MA (US)
Filed on Sep. 7, 2022, as Appl. No. 17/930,338.
Claims priority of provisional application 63/371,978, filed on Aug. 19, 2022.
Claims priority of provisional application 63/274,513, filed on Nov. 1, 2021.
Prior Publication US 2023/0133615 A1, May 4, 2023
Int. Cl. G01N 30/72 (2006.01); G01N 30/86 (2006.01); C12M 1/34 (2006.01); G01N 30/04 (2006.01); G01N 30/02 (2006.01)
CPC G01N 30/72 (2013.01) [C12M 41/30 (2013.01); G01N 30/7233 (2013.01); G01N 30/8665 (2013.01); G01N 2030/027 (2013.01); G01N 2030/045 (2013.01)] 30 Claims
OG exemplary drawing
 
1. A method for high-throughput quantitation of biomolecules, comprising:
(a) combining one or more calibrators with a mixed sample comprising one or more target analytes;
(b) separating the mixed sample using a chromatographic column;
(c) obtaining raw mass spectrometry (MS) data of the separated sample from (b); and
(d) processing the raw MS data using a machine learning model means to directly determine, in less than 12 minutes of total runtime measured from a beginning of the separation in (b), an absolute concentration of the one or more target analytes in the mixed sample.