US 11,753,744 B2
DNA barcoding of designer mononucleosome and chromatin array libraries for the profiling of chromatin readers, writers, erasers, and modulators thereof
Tom W. Muir, Princeton, NJ (US); Uyen T. T. Nguyen, Berlin (DE); and Manuel M. Mueller, London (GB)
Assigned to The Trustees of Princeton University, Princeton, NJ (US)
Filed by The Trustees of Princeton University, Princeton, NJ (US)
Filed on Aug. 24, 2018, as Appl. No. 16/111,603.
Application 16/111,603 is a continuation of application No. 14/405,303, granted, now 10,087,485, previously published as PCT/US2013/044537, filed on Jun. 6, 2013.
Claims priority of provisional application 61/712,148, filed on Oct. 10, 2012.
Claims priority of provisional application 61/656,233, filed on Jun. 6, 2012.
Prior Publication US 2019/0093159 A1, Mar. 28, 2019
Int. Cl. C40B 40/10 (2006.01); C12Q 1/48 (2006.01); G01N 33/68 (2006.01); C40B 30/08 (2006.01); C40B 20/04 (2006.01); C40B 30/04 (2006.01); C12N 15/10 (2006.01); C12Q 1/6876 (2018.01)
CPC C40B 40/10 (2013.01) [C12N 15/1065 (2013.01); C12Q 1/48 (2013.01); C12Q 1/6876 (2013.01); C40B 20/04 (2013.01); C40B 30/04 (2013.01); C40B 30/08 (2013.01); G01N 33/6875 (2013.01)] 30 Claims
OG exemplary drawing
 
1. A library of synthetic mononucleosomes and/or synthetic polynucleosomes, comprising at least two synthetic modified mononucleosome types and/or synthetic modified polynucleosome types,
wherein each synthetic modified mononucleosome type and each synthetic modified polynucleosome type comprises nucleosomal DNA and a histone that is modified to form a pattern of histone modification,
wherein each synthetic modified mononucleosome type and each synthetic modified polynucleosome type comprises a unique DNA barcode inserted at a defined position that is at the 5′- or 3′-end of or within the nucleosomal DNA,
wherein a sequence and/or the defined position of the unique DNA barcode indicates the pattern of histone modification in the histone of each synthetic modified mononucleosome type and each synthetic modified polynucleosome type and no other pattern of histone modification in the histone of another synthetic modified mononucleosome type and/or another synthetic modified polynucleosome type,
wherein the pattern of histone modification in the histone of each synthetic modified mononucleosome type and each synthetic modified polynucleosome type is different from the pattern of histone modification in the histone of each other synthetic modified mononucleosome type and each other synthetic modified polynucleosome type.