CPC C40B 40/10 (2013.01) [C12N 15/1065 (2013.01); C12Q 1/48 (2013.01); C12Q 1/6876 (2013.01); C40B 20/04 (2013.01); C40B 30/04 (2013.01); C40B 30/08 (2013.01); G01N 33/6875 (2013.01)] | 30 Claims |
1. A library of synthetic mononucleosomes and/or synthetic polynucleosomes, comprising at least two synthetic modified mononucleosome types and/or synthetic modified polynucleosome types,
wherein each synthetic modified mononucleosome type and each synthetic modified polynucleosome type comprises nucleosomal DNA and a histone that is modified to form a pattern of histone modification,
wherein each synthetic modified mononucleosome type and each synthetic modified polynucleosome type comprises a unique DNA barcode inserted at a defined position that is at the 5′- or 3′-end of or within the nucleosomal DNA,
wherein a sequence and/or the defined position of the unique DNA barcode indicates the pattern of histone modification in the histone of each synthetic modified mononucleosome type and each synthetic modified polynucleosome type and no other pattern of histone modification in the histone of another synthetic modified mononucleosome type and/or another synthetic modified polynucleosome type,
wherein the pattern of histone modification in the histone of each synthetic modified mononucleosome type and each synthetic modified polynucleosome type is different from the pattern of histone modification in the histone of each other synthetic modified mononucleosome type and each other synthetic modified polynucleosome type.
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