US 11,753,689 B2
Primer set for use in detection of Candida auris, Candida auris detection kit, and Candida auris detection method
Koichi Makimura, Tokyo (JP); Mikachi Yamamoto, Tokyo (JP); and Mohamed Mahdi Alshahni, Tokyo (JP)
Assigned to TEIKYO UNIVERSITY, Tokyo (JP)
Appl. No. 17/48,244
Filed by TEIKYO UNIVERSITY, Tokyo (JP)
PCT Filed Apr. 25, 2019, PCT No. PCT/JP2019/017606
§ 371(c)(1), (2) Date Oct. 16, 2020,
PCT Pub. No. WO2019/208691, PCT Pub. Date Oct. 31, 2019.
Claims priority of application No. 2018-086653 (JP), filed on Apr. 27, 2018.
Prior Publication US 2021/0164058 A1, Jun. 3, 2021
Int. Cl. C12Q 1/68 (2018.01); C12Q 1/6895 (2018.01); C12Q 1/6844 (2018.01)
CPC C12Q 1/6895 (2013.01) [C12Q 1/6844 (2013.01)] 6 Claims
 
1. A primer set for detecting Candida auris by amplifying a Candida auris target sequence in a specimen by the loop-mediated isothermal amplification (LAMP) method, comprising a forward inner primer (FIP), a backward inner primer (BIP), a forward outer primer (F3) and a backward outer primer (B3),
wherein:
FIP is a polynucleotide comprising SEQ ID NO: 1;
BIP is a polynucleotide comprising SEQ ID NO: 2;
F3 is a polynucleotide comprising SEQ ID NO: 3; and
B3 is a polynucleotide comprising SEQ ID NO: 4.