	US 11,753,670 B1
	Cell-free protein synthesis methods using lysates of yersinia pestis
Aleksandr E. Miklos, Baldwin, MD (US); Katherine A. Rhea, Edgewood, MD (US); Nathan D. McDonald, Newark, DE (US); Stephanie D. Cole, Forest Hill, MD (US); and Patricia E. Buckley, Parkville, MD (US)
Assigned to The United States of America as Represented by the Secretary of the Army, Washington, DC (US)
Filed by U.S. Army Combat Capabilities Development Command, Chemical Biological Center, APG, MD (US)
Filed on Jul. 12, 2022, as Appl. No. 17/862,495.
Claims priority of provisional application 63/224,976, filed on Jul. 23, 2021.
Int. Cl. C12P 21/02 (2006.01); C12N 9/12 (2006.01); C12N 15/74 (2006.01)

CPC C12P 21/02 (2013.01) [C12N 9/1247 (2013.01); C12N 15/74 (2013.01); C12Y 207/07006 (2013.01); C12N 2800/101 (2013.01)]

19 Claims

1. A platform for in vitro transcription of mRNA and/or translation of peptides, polypeptides, or sequence defined polymers, the platform comprising:

a cell free extract prepared from a cell culture of a species of Yersinia, and

a transcription template, a translation template, or both a transcription template and a translation template.