US 11,753,665 B2
Method for improving productivity of 2′-fucosyllactose through enzymatic treatment
Chui Soo Shin, Suwon-si (KR); Jong Won Yoon, Seongnam-si (KR); Young Ha Song, Yongin-si (KR); Jong Gil Yoo, Suwon-si (KR); Young Sun Yoo, Hwaseong-si (KR); and Heon Hak Lee, Pyeongtaek-si (KR)
Assigned to Advanced Protein Technologies Corp., Suwon-si (KR); and SEOUL NATIONAL UNIVERSITY R&DB FOUNDATION, Seoul (KR)
Appl. No. 18/246
Filed by Advanced Protein Technologies Corp., Suwon-si (KR); and SEOUL NATIONAL UNIVERSITY R&DB FOUNDATION, Seoul (KR)
PCT Filed Nov. 1, 2022, PCT No. PCT/KR2022/016912
§ 371(c)(1), (2) Date Nov. 29, 2022,
.
Claims priority of application No. 10-2021-0163686 (KR), filed on Nov. 24, 2021.
Prior Publication US 2023/0193334 A1, Jun. 22, 2023
Int. Cl. C12P 19/04 (2006.01); C12N 9/90 (2006.01); C12N 9/88 (2006.01); C12N 9/02 (2006.01); C12N 9/26 (2006.01); C12N 9/04 (2006.01); C12N 9/12 (2006.01); C12N 15/77 (2006.01); C12P 19/00 (2006.01); C12N 9/24 (2006.01); C12N 9/10 (2006.01)
CPC C12P 19/00 (2013.01) [C12N 9/1051 (2013.01); C12N 9/2402 (2013.01); C12Y 204/01069 (2013.01); C12Y 302/01023 (2013.01)] 5 Claims
 
1. A method for preparing 2′-fucosyllactose by culturing recombinant Corynebacterium glutamicum in a medium supplemented with lactose,
wherein the recombinant Corynebacterium glutamicum is transformed to express α-1,2-fucosyltransferase, is transformed to express GDP-D-mannose-4,6-dehydratase, is transformed to express GDP-L-fucose synthase, and is transformed to express lactose permease, and the Corynebacterium glutamicum has phosphomannomutase and GTP-mannose-1-phosphate guanylyltransferase,
wherein beta-galactosidase is fed into the medium in a stationary phase or death phase.