CPC C12P 19/00 (2013.01) [C12N 9/1051 (2013.01); C12N 9/2402 (2013.01); C12Y 204/01069 (2013.01); C12Y 302/01023 (2013.01)] | 5 Claims |
1. A method for preparing 2′-fucosyllactose by culturing recombinant Corynebacterium glutamicum in a medium supplemented with lactose,
wherein the recombinant Corynebacterium glutamicum is transformed to express α-1,2-fucosyltransferase, is transformed to express GDP-D-mannose-4,6-dehydratase, is transformed to express GDP-L-fucose synthase, and is transformed to express lactose permease, and the Corynebacterium glutamicum has phosphomannomutase and GTP-mannose-1-phosphate guanylyltransferase,
wherein beta-galactosidase is fed into the medium in a stationary phase or death phase.
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