US 11,753,656 B2
Methods for the improvement of product yield and production in a microorganism through glycerol recycling
Aaron Argyros, Etna, NH (US); William R. Kenealy, Carlsbad, CA (US); and Emily Stonehouse, Etna, NH (US)
Assigned to LALLEMAND HUNGARY LIQUIDITY MANAGEMENT LLC, Budapest (HU)
Appl. No. 14/911,102
Filed by LALLEMAND HUNGARY LIQUIDITY MANAGEMENT LLC, Budapest (HU)
PCT Filed Aug. 15, 2014, PCT No. PCT/US2014/051355
§ 371(c)(1), (2) Date Feb. 9, 2016,
PCT Pub. No. WO2015/023989, PCT Pub. Date Feb. 19, 2015.
Claims priority of provisional application 61/866,338, filed on Aug. 15, 2013.
Prior Publication US 2016/0194669 A1, Jul. 7, 2016
Int. Cl. C12P 7/06 (2006.01); C12N 9/00 (2006.01); C12N 9/04 (2006.01); C12N 1/22 (2006.01); C07K 14/395 (2006.01); C12N 1/32 (2006.01); C12N 15/81 (2006.01); C12P 7/04 (2006.01)
CPC C12P 7/06 (2013.01) [C07K 14/395 (2013.01); C12N 1/22 (2013.01); C12N 1/32 (2013.01); C12N 9/0006 (2013.01); C12N 15/81 (2013.01); C12P 7/04 (2013.01); C12Y 101/01008 (2013.01); Y02E 50/10 (2013.01)] 25 Claims
 
1. A recombinant yeast comprising:
(a) a heterologous Sugar Transporter-Like protein (STL1); and
(b) a heterologous enzyme comprising an acetaldehyde dehydrogenase and/or a bifunctional acetaldehyde/alcohol dehydrogenase; and
wherein the recombinant yeast produces increased ethanol relative to a control recombinant yeast without said heterologous STL1 protein.