US 11,753,650 B2
Wheat fertility-related gene TaMS7 and application method thereof
Jian Li, Beijing (CN); Zheng Wang, Beijing (CN); Ligeng Ma, Beijing (CN); and Xingwang Deng, Beijing (CN)
Assigned to BEIJING NEXT GENERATION HYBRID WHEAT BIOTECHNOLOGY CO., LTD, Beijing (CN); and PEKING UNIVERSITY INSTITUTE OF ADVANCED AGRICULTURAL SCIENCES, Shandong (CN)
Appl. No. 16/611,863
Filed by FRONTIER LABORATORIES OF SYSTEMS CROP DESIGN CO., LTD., Beijing (CN); and PEKING UNIVERSITY INSTITUTE OF ADVANCED AGRICULTURAL SCIENCES, Shandong (CN)
PCT Filed Nov. 8, 2017, PCT No. PCT/CN2017/109813
§ 371(c)(1), (2) Date Nov. 8, 2019,
PCT Pub. No. WO2018/205521, PCT Pub. Date Nov. 15, 2018.
Claims priority of application No. 201710321452.3 (CN), filed on May 9, 2017.
Prior Publication US 2021/0139931 A1, May 13, 2021
Int. Cl. C12N 15/82 (2006.01)
CPC C12N 15/8289 (2013.01) [C12N 15/8213 (2013.01); C12N 15/8231 (2013.01)] 17 Claims
 
1. A method for propagating a male sterile line of wheat, wherein the method comprises the following steps:
(a) producing a maintainer line by transforming an ms7 male sterile line with a vector that comprises: a fertility restoration gene TaMS7 which can restore the male fertility of the ms7 male sterile line; and a pollen inactivation gene, the expression of which disturbs the function or formation of a male gamete containing the pollen inactivation gene in a plant, so the fertile male gametes generated in the plant do not contain the vector, and a screening gene, which is used for sorting transgenic seeds and non-transgenic seeds; and
(b) selfing the maintainer line plants transformed with the above vector to produce a ms7 male sterile line without the vector and a maintainer line seed containing the vector; or pollinating ms7 male sterile line plants with the pollen grains of the maintainer line plants to propagate the seeds of the ms7 male sterile line;
a nucleotide sequence of the fertility restoring gene TaMS7 is selected from one of the following groups of sequences:
(a) a nucleotide sequence as shown in SEQ ID NO: 1, 2, 3, 4, 5 or 6; or
(b) a nucleotide sequence which codes an amino acid sequence as shown in SEQ ID NO: 7, 8 or 9.