CPC C12N 15/8289 (2013.01) [C12N 15/8213 (2013.01); C12N 15/8231 (2013.01)] | 17 Claims |
1. A method for propagating a male sterile line of wheat, wherein the method comprises the following steps:
(a) producing a maintainer line by transforming an ms7 male sterile line with a vector that comprises: a fertility restoration gene TaMS7 which can restore the male fertility of the ms7 male sterile line; and a pollen inactivation gene, the expression of which disturbs the function or formation of a male gamete containing the pollen inactivation gene in a plant, so the fertile male gametes generated in the plant do not contain the vector, and a screening gene, which is used for sorting transgenic seeds and non-transgenic seeds; and
(b) selfing the maintainer line plants transformed with the above vector to produce a ms7 male sterile line without the vector and a maintainer line seed containing the vector; or pollinating ms7 male sterile line plants with the pollen grains of the maintainer line plants to propagate the seeds of the ms7 male sterile line;
a nucleotide sequence of the fertility restoring gene TaMS7 is selected from one of the following groups of sequences:
(a) a nucleotide sequence as shown in SEQ ID NO: 1, 2, 3, 4, 5 or 6; or
(b) a nucleotide sequence which codes an amino acid sequence as shown in SEQ ID NO: 7, 8 or 9.
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