CPC C12N 9/22 (2013.01) [C12N 15/86 (2013.01); C12N 2750/14143 (2013.01)] | 11 Claims |
1. A polynucleotide comprising a first nucleic acid sequence encoding a first engineered meganuclease and a second nucleic acid sequence encoding a second engineered meganuclease,
wherein said first engineered meganuclease binds and cleaves a nucleic acid at a site that comprises a recognition sequence consisting of the nucleic acid sequence of SEQ ID NO: 6 in a dystrophin gene and comprises the amino acid sequence of SEQ ID NO: 43,
and wherein said second engineered meganuclease binds and cleaves a nucleic acid at a site that comprises a recognition sequence consisting of the nucleic acid sequence of SEQ ID NO: 10 in said dystrophin gene, wherein said second engineered meganuclease comprises the amino acid sequence of SEQ ID NO: 51.
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