CPC C12N 5/0093 (2013.01) [G01N 15/147 (2013.01); G01N 21/6456 (2013.01); G02B 21/16 (2013.01); G01N 2021/646 (2013.01)] | 19 Claims |
1. A method which provides real-time feedback for a system that destroys an undesired cell, comprising:
detecting a first fluorescence of a cell with a detector with a detection event lasting no more than six microseconds (6 μs);
classifying the cell as an undesired cell or a desired cell based on the detection of the first fluorescence;
destroying the undesired cell with a kill event lasting between ten and fifteen nanoseconds (10-15 ns) if the cell is classified as an undesired cell; and
confirming whether the undesired cell was destroyed by detecting a second fluorescence of the cell during the detection event.
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