US 11,753,620 B2
Apparatus and method for cell kill confirmation
Frederick Savage, DeForest, WI (US); David Appleyard, DeForest, WI (US); Zheng Xia, DeForest, WI (US); Matthew Ebersole, DeForest, WI (US); and Daniel McAda, DeForest, WI (US)
Assigned to ABS Global, Inc., DeForest, WI (US)
Filed by ABS Global, Inc., DeForest, WI (US)
Filed on Jul. 13, 2022, as Appl. No. 17/812,369.
Application 17/812,369 is a continuation of application No. 16/442,058, filed on Jun. 14, 2019, granted, now 11,427,804.
Claims priority of provisional application 62/685,614, filed on Jun. 15, 2018.
Prior Publication US 2022/0348865 A1, Nov. 3, 2022
Int. Cl. G01N 15/14 (2006.01); C12N 5/00 (2006.01); G01N 21/64 (2006.01); G02B 21/16 (2006.01)
CPC C12N 5/0093 (2013.01) [G01N 15/147 (2013.01); G01N 21/6456 (2013.01); G02B 21/16 (2013.01); G01N 2021/646 (2013.01)] 19 Claims
OG exemplary drawing
 
1. A method which provides real-time feedback for a system that destroys an undesired cell, comprising:
detecting a first fluorescence of a cell with a detector with a detection event lasting no more than six microseconds (6 μs);
classifying the cell as an undesired cell or a desired cell based on the detection of the first fluorescence;
destroying the undesired cell with a kill event lasting between ten and fifteen nanoseconds (10-15 ns) if the cell is classified as an undesired cell; and
confirming whether the undesired cell was destroyed by detecting a second fluorescence of the cell during the detection event.