Method for propagating microorganisms within plant bioreactors and stably storing microorganisms within agricultural seeds
Birgit Mitter, Giesshubl (AT); Muhammad Naveed, Faisalabad (PK); Teresa Berninger, Vienna (AT); Stephane Compant, Vienna (AT); Angela Sessitsch, Vienna (AT); Geoffrey Von Maltzahn, Boston, CA (US); Richard Bailey Flavell, Thousand Oaks, CA (US); Gerardo V. Toledo, Belmont, MA (US); Slavica Djonovic, Malden, MA (US); Luis Miguel Marquez, Belmont, MA (US); David Morris Johnston, Cambridge, MA (US); Yves Alain Millet, Newtonville, MA (US); Jeffrey Lyford, Hollis, NH (US); Jonathan W. Leff, Cambridge, MA (US); Phillip Samayoa, Cambridge, MA (US); and Craig Sadowski, Somerville, MA (US)
Assigned to Indigo Ag, Inc., Boston, MA (US); and AIT Austrian Institute of Technology GMBH, Vienna (AT)
Filed by Indigo Ag, Inc., Boston, MA (US); and AIT AUSTRIAN INSTITUTE OF TECHNOLOGY GMBH, Vienna (AT)
Filed on Dec. 17, 2020, as Appl. No. 17/124,955.
Application 17/124,955 is a continuation of application No. 16/512,219, filed on Jul. 15, 2019, granted, now 10,932,469.
Application 16/512,219 is a continuation of application No. 15/107,965, granted, now 10,362,787, issued on Jul. 30, 2019, previously published as PCT/US2014/072400, filed on Dec. 24, 2014.
Application 15/107,965 is a continuation of application No. 14/315,804, filed on Jun. 26, 2014, granted, now 9,364,005.
Claims priority of provisional application 61/920,560, filed on Dec. 24, 2013.
Claims priority of provisional application 62/017,816, filed on Jun. 26, 2014.
Claims priority of provisional application 62/017,809, filed on Jun. 26, 2014.
Claims priority of provisional application 62/017,813, filed on Jun. 26, 2014.
Claims priority of provisional application 62/017,796, filed on Jun. 26, 2014.
Claims priority of provisional application 62/017,818, filed on Jun. 26, 2014.
Claims priority of provisional application 62/017,815, filed on Jun. 26, 2014.
Claims priority of application No. PCT/US2014/044427 (WO), filed on Jun. 26, 2014; and application No. PCT/US2014/054160 (WO), filed on Sep. 4, 2014.
Prior Publication US 2021/0363480 A1, Nov. 25, 2021
1. A method of producing a substance selected from the group consisting of a putative peptidyl-prolyl cis-trans isomerase, ATP-dependent Clp protease proteolytic subunit, and translation elongation factor Tu isoform 3, within a bacterial colonized cereal plant bioreactor, the method comprising:
a) germinating a cereal seed into which at least one inoculant bacterial endophyte has been introduced, wherein the bacterial endophyte is from the genus Burkholderia, comprises a 16S rRNA nucleic acid sequence comprising SEQ ID NO: 1446 and can grow on nitrogen free media, to produce the bacterial colonized cereal plant bioreactor; and
b) growing the bacterial colonized cereal plant bioreactor under conditions such that the bacterial endophyte proliferates and the substance is produced in the bacterial colonized cereal plant bioreactor, wherein the substance improves growth.