US 11,753,613 B2
Methods and devices for generating chemical and gaseous gradients in microfluidic platforms
Nitin Agrawal, Fairfax, VA (US)
Assigned to Children's National Medical Center, Washington, DC (US)
Filed by Children's National Medical Center, Washington, DC (US)
Filed on Nov. 7, 2018, as Appl. No. 16/183,204.
Claims priority of provisional application 62/582,394, filed on Nov. 7, 2017.
Prior Publication US 2019/0136177 A1, May 9, 2019
Int. Cl. C12M 1/34 (2006.01); C12M 3/06 (2006.01); C12M 1/12 (2006.01); C12M 1/04 (2006.01); C12N 5/00 (2006.01); C12M 1/107 (2006.01); C12M 1/00 (2006.01)
CPC C12M 41/34 (2013.01) [C12M 23/16 (2013.01); C12M 23/20 (2013.01); C12M 23/24 (2013.01); C12M 23/34 (2013.01); C12M 23/36 (2013.01); C12M 25/02 (2013.01); C12M 41/32 (2013.01); C12N 5/0075 (2013.01)] 8 Claims
OG exemplary drawing
 
1. A microfluidic device, comprising:
a glass or polymethylmethacrylate substrate; and
a layer of polydimethylsiloxane (PDMS) on and bonded to the substrate, the layer of PDMS comprising at least one reservoir having an inner wall of PDMS, the reservoir comprising:
at least two media inlets;
at least one cell chamber;
at least one cell inlet connected to the cell chamber;
at least one serpentine mixing channel network from the at least two media inlets to the cell chamber; and
at least one media outlet connected to the cell chamber;
wherein
the at least one serpentine mixing channel network is configured to provide a media gradient across the cell chamber, and
the inner PDMS wall of the at least one serpentine mixing channel network is coated with a glass substance that inhibits diffusion of a gas within the channel network.