CPC C07K 1/22 (2013.01) [C07K 16/02 (2013.01); G01N 33/569 (2013.01)] | 6 Claims |
1. A kit for detecting Giardia antigens consisting of the following components: a first capture consisting of purified polyclonal anti-Giardia IgG or anti-Giardia IgY antibodies, and a second capture consisting of purified polyclonal anti-Giardia IgY or anti-Giardia IgG antibodies; provided that the first capture and the second capture are not the same; a solid matrix selected from polystyrene plates or nitrocellulose membrane (MNC); a blocking solution; phosphate buffer saline −0.1% Polysorbate 20 pH 7.2 (PBS-T); a conjugate of anti-IgY or anti-IgG antibodies bound to a reporter; and optionally a detection reagent; wherein if the second capture is a polyclonal anti-Giardia IgY antibody, the conjugate is a conjugate of anti-IgY; or wherein if the second capture is a polyclonal anti-Giardia IgG antibody, and the conjugate is a conjugate of anti-IgG; and wherein the purified polyclonal anti-Giardia IgG antibodies and the polyclonal anti-Giardia IgY antibodies are purified by affinity chromatography with the recombinant Giardia antigens Alpha Giardin 7.3, Kinesin 3 and Cyst wall protein 1 (CWP1).
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