CPC C07K 1/165 (2013.01) [G01N 33/5002 (2013.01)] | 8 Claims |
1. A method of purifying peptides and/or polypeptides bound to mixed- phase SPE material, said method comprising:
(a) loading a sample comprising peptides and/or polypeptides under acidic or neutral aqueous conditions on mixed-phase solid phase extraction (SPE) material comprising reversed phase material and/or ion exchange material;
(b) washing with
(ba) an acidic composition having a pH from 1 to 4 and comprising at least 50% (v/v) organic solvent; and
(bb) an acidic or neutral aqueous solution; and
(c) eluting the peptides and/or polypeptides from said mixed-phase SPE material with an alkaline composition comprising at least 50% (v/v) organic solvent;
wherein said mixed-phase SPE material is sulfonated poly-divinyl benzene (DVB) or sulfonated poly-styrene divinyl benzene (SDB); and
wherein step (a) is performed prior to step (b) which is performed prior to step (c).
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