	US 11,752,335 B1
	Method for determining facial muscle responses
Shoná Burkes-Henderson, Dallas, TX (US); Geetha Kalahasti, Plano, TX (US); and David Gan, Southlake, TX (US)
Assigned to MARY KAY INC., Addison, TX (US)
Filed by Mary Kay Inc., Addison, TX (US)
Filed on Feb. 7, 2019, as Appl. No. 16/270,211.
Claims priority of provisional application 62/627,279, filed on Feb. 7, 2018.
Int. Cl. A61N 1/36 (2006.01); A61B 5/00 (2006.01); A61K 38/48 (2006.01); A61Q 19/08 (2006.01); A61N 1/04 (2006.01); A61K 49/00 (2006.01)

CPC A61N 1/36014 (2013.01) [A61B 5/4519 (2013.01); A61K 38/4893 (2013.01); A61Q 19/08 (2013.01); A61K 49/0006 (2013.01); A61N 1/0452 (2013.01)]

13 Claims

1. A method for quantitatively assessing muscle contraction of a facial muscle in a person and determining the ability of a treatment material to reduce contraction of the facial muscle, the method comprising:

(a) applying an external electrical stimulus of 2 mV to 10 mV of electricity at 2 to 4 second intervals for 5 to 15 intervals to facial skin sufficient to contract a facial muscle; and

(b) measuring the contractile activity of the contracted facial muscle by measuring the compound motor action potential (CMAP) of the contracted facial muscle to obtain a first average measurement of contractile activity of the contracted facial muscle; and then

(d) repeating steps (a) and (b) to obtain a second average measurement of contractile activity of the contracted facial muscle,

wherein a decrease in contractile activity based on the second average measurement as compared to the first average measurement indicates that the treatment material reduces contraction of the facial muscle when administered to the facial skin, and

wherein the method for quantitatively assessing muscle contraction of the facial muscle does not rely on the use of voluntary contractions of the facial muscle by the person.