	US 11,752,168 B2
	Methods of using cosmetic compositions comprising exopolysaccharides derived from microbial mats
Estelle Loing, Québec (CA); Sandrine Briatte, Saint-Rédempteur (CA); Patrice Dionne, Saint-Rédempteur (CA); Martin Beaulieu, Rimouski (CA); Xavier Moppert, Punaauia (PF); Laurent Richert, Pirac (PF); and Catherine Vayssier, Québec (CA)
Assigned to LUCAS MEYER COSMETICS CANADA INC., Quebec (CA)
Filed by Lucas Meyer Cosmetics Canada Inc., Québec (CA)
Filed on May 12, 2021, as Appl. No. 17/302,784.
Application 17/302,784 is a division of application No. 15/979,011, filed on May 14, 2018, granted, now 11,040,058.
Application 15/979,011 is a continuation of application No. 12/988,211, abandoned, previously published as PCT/CA2009/000493, filed on Apr. 15, 2009.
Claims priority of provisional application 61/044,992, filed on Apr. 15, 2008.
Prior Publication US 2021/0330691 A1, Oct. 28, 2021
Int. Cl. A61K 31/715 (2006.01); A61K 8/73 (2006.01); A61K 8/99 (2017.01); A61Q 19/08 (2006.01)

CPC A61K 31/715 (2013.01) [A61K 8/73 (2013.01); A61K 8/99 (2013.01); A61Q 19/08 (2013.01)]

14 Claims

1. A method of reducing at least one skin aging sign in a subject comprising administering on the skin of the subject an effective amount of a composition comprising at least one isolated native exopolysaccharide (EPS), wherein the at least one EPS comprises Pol-6 and Pol-5, the at least one EPS being in a concentration of about 0.001% w/w to about 1.5% w/w of the composition, wherein:

a) said Pol-6:

i) has a molar ratio of about 0.7 rhamnose and 0.7 mannose for a glucose reference of 10.0, as determined by gas chromatography;

ii) does not comprise galactose, xylose, and galacturonic acid, as determined by gas chromatography; and

iii) is secreted by a Vibrio alginolyticus bacterial strain originally isolated from a kopara microbial mat; and

b) said Pol-5:

i) has a molar ratio of about 3.2 D-glucose, 2.9 D-galactose, 0.5 D-mannose, 2.0 D-glucuronic acid and 1.0 D-galacturonic acid, as determined by gas chromatography; and

ii) is secreted by an Alteromonas macleodii bacterial strain originally isolated from a kopara microbial mat;

wherein at least one skin aging sign is reduced.