	US 11,749,378 B2
	Methods and devices for nucleic acid-based real-time determination of disease states
Philip Stevens, Alpen (DE)
Assigned to Noscendo GmbH, Duisburg (DE)
Appl. No. 16/631,523
Filed by Noscendo GmbH, Duisburg (DE)
PCT Filed Jul. 18, 2018, PCT No. PCT/EP2018/069493 § 371(c)(1), (2) Date Jan. 16, 2020, PCT Pub. No. WO2019/016258, PCT Pub. Date Jan. 24, 2019.
Claims priority of application No. 17182104 (EP), filed on Jul. 19, 2017.
Prior Publication US 2020/0176079 A1, Jun. 4, 2020
Int. Cl. A61B 5/00 (2006.01); A61B 10/00 (2006.01); C12Q 1/68 (2018.01); C12Q 1/06 (2006.01); C12Q 1/04 (2006.01); G16B 30/00 (2019.01); C12Q 1/689 (2018.01); G16B 35/00 (2019.01)

CPC G16B 30/00 (2019.02) [C12Q 1/689 (2013.01); C12Q 2535/122 (2013.01); G16B 35/00 (2019.02)]

15 Claims

1. A method of treating a disease or infection caused by a microorganism in a subject comprising administering to the subject a compound that inhibits the growth of a microorganism whose significance score meets or exceeds a threshold value, wherein the significance score is calculated by:

comparing sequence reads with one or more databases comprising the genetic information from a control subject of the same species and the genetic information from a plurality of microorganisms to determine whether or not a compared sequence read maps to a species comprised within the one or more databases, wherein the sequence reads are obtained by sequencing nucleic acids present in a biological sample obtained from the subject;

determining over time the number of compared sequence reads mapping to a particular microorganism and the number of compared sequence reads mapping to a species, wherein the sequence reads mapping to a species comprise sequence reads mapping to the species of the subject; and

computing a significance score for the probability of finding in the subject a compared sequence read mapping to the particular microorganism based on the number of compared sequence reads mapping to the particular microorganism and the number of compared sequence reads mapping to a species.