CPC G01N 33/948 (2013.01) [A61B 10/00 (2013.01); B01L 3/5027 (2013.01); B01L 3/50273 (2013.01); B01L 3/502715 (2013.01); B01L 3/502738 (2013.01); B01L 3/502746 (2013.01); G01N 21/6428 (2013.01); G01N 21/766 (2013.01); G01N 33/497 (2013.01); G01N 33/4972 (2013.01); G01N 33/542 (2013.01); G01N 33/54306 (2013.01); G01N 33/98 (2013.01); A61B 2010/0009 (2013.01); A61B 2010/0087 (2013.01); B01L 2300/02 (2013.01); B01L 2300/0636 (2013.01); B01L 2400/049 (2013.01); B01L 2400/0638 (2013.01)] | 14 Claims |
1. A method for evaluating tetrahydrocannabinol (THC) level in a breath sample, the method comprising:
drawing a portion of a breath sample of a subject into a reaction channel of a test cartridge, the test cartridge comprising a microfluidic device having one or more reaction channel walls on which a THC antibody is surface-bound, wherein THC from the portion of the breath sample drawn into the reaction channel is captured by binding to the THC antibody;
determining an amount of THC captured from the portion of the breath sample by:
flowing a diazotized fluorophore into the reaction channel and forming a solution such that the diazotized fluorophore binds to any THC from the portion of the breath sample captured by binding to the THC antibody, to form a diazotized fluorophore-THC adduct;
exposing the diazotized fluorophore-THC adduct to a light source to produce a fluorescence;
measuring an intensity of the fluorescence; and
determining the amount of THC captured from the portion of the breath sample based on the measured intensity of the fluorescence;
comparing the determined amount of THC captured from the portion of the breath sample to a threshold level for THC in breath, wherein the threshold level is based on a measured intensity of fluorescence from similar measurements performed for a control amount of THC; and
indicating whether or not the determined amount of THC captured from the portion of the breath sample exceeds the threshold level using a visible and/or audible signal and/or readout on a display associated with a device on which the determining and comparing is performed.
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