US 11,747,353 B1
Analyte detection from breath samples
Michael Scott Lynn, Piedmont, CA (US); Joseph A. Heanue, Fremont, CA (US); Samartha G. Anekal, Fremont, CA (US); Kevin M. Limtao, Milpitas, CA (US); Kevin Bradford Dunk, Castro Valley, CA (US); and Jeffrey A. Schuster, Oakland, CA (US)
Assigned to Hound Labs, Inc., Oakland, CA (US)
Filed by Hound Labs, Inc., Oakland, CA (US)
Filed on Nov. 16, 2021, as Appl. No. 17/455,187.
Application 17/455,187 is a division of application No. 16/124,181, filed on Sep. 6, 2018, granted, now 11,187,711.
Claims priority of provisional application 62/646,798, filed on Mar. 22, 2018.
Claims priority of provisional application 62/616,380, filed on Jan. 11, 2018.
Claims priority of provisional application 62/557,060, filed on Sep. 11, 2017.
Claims priority of provisional application 62/557,056, filed on Sep. 11, 2017.
Int. Cl. G01N 33/94 (2006.01); A61B 10/00 (2006.01); G01N 33/543 (2006.01); G01N 33/542 (2006.01); G01N 33/497 (2006.01); G01N 33/98 (2006.01); B01L 3/00 (2006.01); G01N 21/76 (2006.01); G01N 21/64 (2006.01)
CPC G01N 33/948 (2013.01) [A61B 10/00 (2013.01); B01L 3/5027 (2013.01); B01L 3/50273 (2013.01); B01L 3/502715 (2013.01); B01L 3/502738 (2013.01); B01L 3/502746 (2013.01); G01N 21/6428 (2013.01); G01N 21/766 (2013.01); G01N 33/497 (2013.01); G01N 33/4972 (2013.01); G01N 33/542 (2013.01); G01N 33/54306 (2013.01); G01N 33/98 (2013.01); A61B 2010/0009 (2013.01); A61B 2010/0087 (2013.01); B01L 2300/02 (2013.01); B01L 2300/0636 (2013.01); B01L 2400/049 (2013.01); B01L 2400/0638 (2013.01)] 14 Claims
OG exemplary drawing
 
1. A method for evaluating tetrahydrocannabinol (THC) level in a breath sample, the method comprising:
drawing a portion of a breath sample of a subject into a reaction channel of a test cartridge, the test cartridge comprising a microfluidic device having one or more reaction channel walls on which a THC antibody is surface-bound, wherein THC from the portion of the breath sample drawn into the reaction channel is captured by binding to the THC antibody;
determining an amount of THC captured from the portion of the breath sample by:
flowing a diazotized fluorophore into the reaction channel and forming a solution such that the diazotized fluorophore binds to any THC from the portion of the breath sample captured by binding to the THC antibody, to form a diazotized fluorophore-THC adduct;
exposing the diazotized fluorophore-THC adduct to a light source to produce a fluorescence;
measuring an intensity of the fluorescence; and
determining the amount of THC captured from the portion of the breath sample based on the measured intensity of the fluorescence;
comparing the determined amount of THC captured from the portion of the breath sample to a threshold level for THC in breath, wherein the threshold level is based on a measured intensity of fluorescence from similar measurements performed for a control amount of THC; and
indicating whether or not the determined amount of THC captured from the portion of the breath sample exceeds the threshold level using a visible and/or audible signal and/or readout on a display associated with a device on which the determining and comparing is performed.