CPC G01N 33/86 (2013.01) [C12N 9/644 (2013.01); G01N 2333/755 (2013.01); G01N 2333/9645 (2013.01); G01N 2333/974 (2013.01)] | 15 Claims |
1. A method of quantifying an amount of protein capable of exhibiting FIX activity which is in its activated form (activated FIX protein) in a test sample, the method comprising:
(i) measuring thrombin generation activity for the test sample in the presence of FIX-deficient plasma or FIX-deficient blood and in the presence of exogenous thrombin, wherein the exogenous thrombin is present at a concentration of about 5 nM, and
(ii) comparing the thrombin generation activity to a standard curve plotting known activated FIX protein levels to thrombin generation activity;
wherein the measuring is performed in the absence of exogenous tissue factor (TF).
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