US 11,747,344 B2
Microorganism identification method
Hiroto Tamura, Kani (JP); Naomi Yamamoto, Nagoya (JP); Teruyo Kato, Aisai (JP); Keisuke Shima, Kyoto (JP); and Shinji Funatsu, Kyoto (JP)
Assigned to SHIMADZU CORPORATION, Kyoto (JP); and MEIJO UNIVERSITY, Nagoya (JP)
Appl. No. 16/89,836
Filed by SHIMADZU CORPORATION, Kyoto (JP); and MEIJO UNIVERSITY, Nagoya (JP)
PCT Filed Mar. 31, 2016, PCT No. PCT/JP2016/060865
§ 371(c)(1), (2) Date Sep. 28, 2018,
PCT Pub. No. WO2017/168740, PCT Pub. Date Oct. 5, 2017.
Prior Publication US 2020/0300862 A1, Sep. 24, 2020
Int. Cl. G01N 33/68 (2006.01); C12Q 1/04 (2006.01); G01N 27/64 (2006.01)
CPC G01N 33/6851 (2013.01) [C12Q 1/04 (2013.01); G01N 27/64 (2013.01); G01N 2333/255 (2013.01)] 1 Claim
 
1. A method of identifying Orion or Rissen among serovars of Salmonella bacteria comprising
a) a step of subjecting a sample containing serovars of Salmonella bacteria to mass spectrometry to obtain a mass spectrum,
b) a step of reading a mass-to-charge ratio m/z of a peak derived from a marker protein from the mass spectrum, and
c) an identification step of identifying Salmonella Orion or Salmonella Rissen in the sample, based on the mass-to-charge ratio m/z,
wherein the serovars of Salmonella bacteria comprise one or more of Typhimurium, Enteritidis, Gailinarum Pullorum, Minnesota, Abony, Choleraesuis, UN_O7, Braenderup, Pakistan, Infantis, Thompson, Saintpaul, Brandenburg, Orion, Rissen, Mbandaka, Altona, Istanbul, Senftenberg, Montevideo, UN_O13, UN_O1,3,19, UN_O18, UN_O7, Amsterdam, Manhattan O6,8:d1.5, and Schwarzengrund O4:d:1,7,
wherein when the marker protein comprises peptidylprolyl isomerase, the serovar of Salmonella bacteria is identified as Orion, and
wherein when the marker protein comprises the ribosomal protein S8, the serovar of Salmonella bacteria is identified as Rissen.