US 11,747,339 B2
Materials and methods for performing histochemical assays for human pro-epiregulin and amphiregulin
Fernando Jose Rebelo do Couto, Pleasanton, CA (US); Zhiming Liao, Livermore, CA (US); Andrea Muranyi, Tucson, AZ (US); Kandavel Shanmugam, Oro Valley, AZ (US); Shalini Singh, Tucson, AZ (US); and Yifei Zhu, San Jose, CA (US)
Assigned to Ventana Medical Systems, Inc., Tucson, AZ (US)
Filed by Ventana Medical Systems, Inc., Tucson, AZ (US)
Filed on Oct. 21, 2020, as Appl. No. 16/949,252.
Application 16/949,252 is a division of application No. 15/851,502, filed on Dec. 21, 2017, granted, now 10,852,304.
Application 15/851,502 is a continuation of application No. PCT/EP2016/064883, filed on Jun. 27, 2016.
Claims priority of provisional application 62/186,251, filed on Jun. 29, 2015.
Prior Publication US 2021/0063403 A1, Mar. 4, 2021
Int. Cl. C07K 16/00 (2006.01); G01N 33/574 (2006.01); C07K 16/22 (2006.01); C07K 16/30 (2006.01)
CPC G01N 33/57496 (2013.01) [C07K 16/22 (2013.01); C07K 16/30 (2013.01); C07K 2317/20 (2013.01); C07K 2317/34 (2013.01); C07K 2317/92 (2013.01); G01N 2333/485 (2013.01)] 20 Claims
 
1. An antibody or antibody fragment capable of specifically binding to amino acids 156-169 of SEQ ID NO: 1, wherein the antibody comprises a heavy chain comprising the following hypervariable regions (HVRs):
(a) an HVR-H1 comprising SEQ ID NO: 18;
(b) an HVR-H2 comprising SEQ ID NO: 19; and
(c) an HVR-H3 comprising SEQ ID NO: 20; and
wherein the antibody or antibody fragment further comprises a light chain comprising the following HVRs:
(h) an HVR-L1 comprising an amino acid sequence selected from the group consisting of SEQ ID NO: 25 and SEQ ID NO: 26;
(i) an HVR-L2 comprising SEQ ID NO: 27; and
(j) an HVR-L3 comprising SEQ ID NO: 28.