US 11,747,333 B2
Detection method of target analyte using gold nanoprobe through overgrowth of copper crystal
Jwa-Min Nam, Seoul (KR); Gwangpyo Ko, Seoul (KR); Jae-Ho Kim, Seoul (KR); Jeong-Eun Park, Seoul (KR); Mouhong Lin, Seoul (KR); In Sun Joo, Chungcheongbuk-do (KR); and Jeong Su Lee, Chungcheongbuk-do (KR)
Assigned to KOREA FOOD & DRUG ADMINISTRATION, Chungcheongbuk-Do (KR); and SEOUL NATIONAL UNIVERSITY R&DB FOUNDATION, Seoul (KR)
Appl. No. 16/486,674
Filed by SEOUL NATIONAL UNIVERSITY R&DB FOUNDATION, Seoul (KR); and KOREA FOOD & DRUG ADMINISTRATION, Chungcheongbuk-do (KR)
PCT Filed Feb. 14, 2018, PCT No. PCT/KR2018/001956
§ 371(c)(1), (2) Date Sep. 18, 2019,
PCT Pub. No. WO2018/151542, PCT Pub. Date Aug. 23, 2018.
Claims priority of application No. 10-2017-0021136 (KR), filed on Feb. 16, 2017.
Prior Publication US 2020/0011864 A1, Jan. 9, 2020
Int. Cl. G01N 33/553 (2006.01); G01N 33/543 (2006.01); G01N 33/532 (2006.01); B82Y 30/00 (2011.01)
CPC G01N 33/553 (2013.01) [G01N 33/532 (2013.01); G01N 33/54346 (2013.01); G01N 33/54366 (2013.01); G01N 33/54393 (2013.01); B82Y 30/00 (2013.01)] 15 Claims
OG exemplary drawing
 
1. A composition for amplifying an optical scattering signal from a biological material labelled with a gold nanoparticle, the optical scattering signal generated in response to a light delivered to the biological material, the composition comprising:
a copper ion in solution;
a polymer comprising a primary or a secondary amine group, the primary or the secondary amine group reacts with the copper ion, wherein said polymer comprising a primary or a secondary amine group is polyethyleneimine;
a reducing agent, wherein said reducing agent is ascorbic acid, hydroxylamine or hydroquinone; and
the gold nanoparticle,
wherein a copper crystal is specifically presented on a surface of the gold nanoparticle, and said copper crystal is formed by a reaction of the copper ion and the polymer comprising a primary or a secondary amine group and the reducing agent.