CPC G01N 33/5091 (2013.01) [G01N 1/30 (2013.01); G01N 33/6854 (2013.01); G01N 2800/347 (2013.01)] | 24 Claims |
1. A method for diagnosing or pre-diagnosing a disease associated with podocyte foot process effacement in a subject or for determining the risk of a subject to develop a disease associated with podocyte foot process effacement, said method comprising the steps of
a) determining the length of the slit diaphragm (ISD) formed by podocyte foot processes in a specific area A in a renal tissue sample of said subject by super resolution light microscopy; wherein the specific area A is an area where the glomeruli are visible with a plane view on podocyte foot process;
b) comparing the length of the slit diaphragm (ISD) formed by podocyte foot processes in a specific area A as determined in step (a) with the length of the slit diaphragm (ISD) formed by podocyte foot processes in a comparable specific area A′ in a renal tissue sample of a subject who at the time of the sampling showed no clinical symptoms of a disease associated with podocyte foot process effacement, wherein a deviation indicates the disease associated with podocyte foot process effacement;
wherein
the deviation according to (b) is a decrease of the length of the slit diaphragm (ISD) as determined in (a) compared to the length of the slit diaphragm (ISD) of a subject who at the time of the sampling showed no clinical symptoms of a disease associated with podocyte foot process effacement.
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